CD80/B7-1 Rabbit Polyclonal Antibody
cat.: 1007-8
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: 60 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to Human CD80 / B7-1 aa 71-120 / 288.
Positive control: Human brain tissue lysates, N2A, SHG-44, human tonsil tissue, mouse lung tissue, mouse spleen tissue, Hela.
Subcellular location: Cell membrane
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P33681 Human
Alternative names: Activation B7-1 antigen B lymphocyte activation antigen B7 B7 B7-1 B7-1 antigen B7.1 BB1 CD28 antigen ligand 1 CD28LG CD28LG1 CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) CD80 antigen CD80 molecule CD80_HUMAN Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80
Images
1007-8_1.jpg Fig1: Western blot analysis of CD80 on human brain tissue lysates using anti-CD80 antibody at 1/1,000 dilution.
1007-8_2.jpg Fig2: ICC staining CD80 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
1007-8_3.jpg Fig3: ICC staining CD80 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
1007-8_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD80 antibody. Counter stained with hematoxylin.
1007-8_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-CD80 antibody. Counter stained with hematoxylin.
1007-8_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD80 antibody. Counter stained with hematoxylin.
1007-8_7.jpg Fig7: Flow cytometric analysis of Hela cells with CD80 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.