Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, ICC, IF, ELISA |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 33 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide corresponding to Human CD80 / B7-1 aa 71-120 / 288. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IHC-P ICC IF ELISA |
1:500-1:2,000 1:100-1:500 1:100 1:100-1:500 1:5,000-10,000 |
Uniprot #: | SwissProt: P33681 Human | Q00609 Mouse |
Alternative names: | Activation B7-1 antigen B lymphocyte activation antigen B7 B7 B7-1 B7-1 antigen B7.1 BB1 CD28 antigen ligand 1 CD28LG CD28LG1 CD80 CD80 antigen (CD28 antigen ligand 1, B7-1 antigen) CD80 antigen CD80 molecule CD80_HUMAN Costimulatory factor CD80 costimulatory molecule variant IgV-CD80 CTLA-4 counter-receptor B7.1 LAB7 T-lymphocyte activation antigen CD80 |
Fig1:
Sample: Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug Lane 3: Thymus (Rat) Lysate at 40 ug Lane 4: Raji (Human) Cell Lysate at 30 ug Lane 5: K562 (Human) Cell Lysate at 30 ug Primary: Anti-CD80 (1007-8) at 1/1,000 dilution Secondary: Goat Anti-Rabbit IgG at 1/20,000 dilution Predicted band size: 33 kD Observed band size: 60 kD |