HRP Conjugated V5 tag Rabbit Polyclonal Antibody
cat.: 1008-4
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Species independent |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide immune sequence is GKPIPNPLLGLDSTC. |
| Positive control: | Recombinant protein. |
| Recommended Dilutions:
WB |
1:10,000～1:100,000 |
| Alternative names: | GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST tag Protein Rev Regulator of expression of viral proteins rev V5 epitope tag |
Images
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Fig1:
Western blot analysis of V5 tag on 293T-OE lysates with Rabbit anti-V5 tag antibody (1008-4) at 1/100,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1008-4, 1/100,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.