Beta tubulin Mouse Monoclonal Antibody [1-B11]
cat.: EM0103
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: 1-B11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 50 kDa
Isotype: IgG1
Immunogen: peptide
Positive control: NCCIT, NIH/3T3, PC12, Mouse heart, F9, zebrafish brain, Hela HepG2, Mouse brain
Subcellular location: Cytoplasm ,cytoskeleton
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:5,000-1:10,000
1:200
1:20
1:50-1:100
Uniprot #: SwissProt: P07437 Human
Alternative names: Beta 4 tubulin Beta 5 tubulin BetaTubulin TBB5_HUMAN TUBB TUBB2 TUBB2A TUBB5 tubulin beta 2A Tubulin beta chain Tubulin beta-5 chain
Images
EM0103_1.jpg Fig1: Western blot analysis of β-tubulin on different cell lysates using anti-β-tubulin antibody at 1/5000 dilution.
Positive control:
Lane 1: NCCIT
Lane 2: NIH/3T3
Lane 3: PC12
Lane 4: Mouse heart
Lane 5: F9
Lane 6: zebrafish brain
Lane 7: Hela
EM0103_2.jpg Fig2: ICC staining β-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM0103_3.jpg Fig3: ICC stainingβ-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM0103_4.jpg Fig4: ICC stainingβ-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM0103_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin.
EM0103_6.jpg Fig6: Flow cytometric analysis of HeLa cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
EM0103_7.jpg Fig7: Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/500 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.