beta Tubulin Mouse Monoclonal Antibody [1-B11]
cat.: EM0103
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 1-B11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Beta tubulin aa 51-100. |
| Positive control: | K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, Hela, HepG2, NIH/3T3, mouse brain tissue, hybrid fish (crucian-carp) brain tissue lysates. |
| Subcellular location: | Cytoplasm ,cytoskeleton |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000-1:20,000 1:1,000 1:20 1:50-1:100 |
| Uniprot #: | SwissProt: P07437 Human | P99024 Mouse | P69897 Rat |
| Alternative names: | Beta 4 tubulin Beta 5 tubulin BetaTubulin TBB5_HUMAN TUBB TUBB2 TUBB2A TUBB5 tubulin beta 2A Tubulin beta chain Tubulin beta-5 chain |
Images
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Fig1:
Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (EM0103) at 1/20,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0103) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Hela cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (EM0103) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (EM0103) at 1/1,000 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig3:
Immunocytochemistry analysis of HepG2 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (EM0103) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (EM0103) at 1/1,000 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (EM0103) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (EM0103) at 1/500 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-β-tubulin antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of Hela cells with β-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
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Fig7: Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/500 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.