EM010805

Product Type:	Mouse monoclonal IgM, primary antibodies
Species reactivity:	Human
Applications:	WB, IHC-P, FC
Clonality:	Monoclonal
Clone number:	A7-1

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

Predicted band size: 81 kDa

Isotype:

IgM

Immunogen:

Synthetic peptide within human CD44 aa 100-200.

Positive control:

HUVEC cell lysate, HL-60 cell lysate, Hela cell lysate, SKOV-3 cell lysate, human tonsil tissue, human colon carcinoma tissue, Hela.

Subcellular location:

Cell membrane

Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:200
1:100-1:200

Uniprot #:

SwissProt: P16070 Human

Alternative names:

LHR BA-1 CD 44 CD44 CD44 antigen CD44 molecule (Indian blood group) CD44 molecule CD44_HUMAN CDw44 Cell surface glycoprotein CD44 chondroitin sulfate proteoglycan 8 CSPG8 ECMR-III Epican Extracellular matrix receptor III GP90 lymphocyte homing/adhesion receptor HCELL hematopoietic cell E- and L-selectin ligand Heparan sulfate proteoglycan Hermes antigen homing function and Indian blood group system HSA HUTCH-I HUTCH1 Hyaluronate receptor IN INLU-related p80 Glycoprotein MC56 MDU2 MDU3 MGC10468 MIC4 MUTCH1 PGP-1 PGP-I PGP1 Phagocytic glycoprotein 1 Phagocytic glycoprotein I Soluble CD44

	Fig1: Western blot analysis of CD44 on different lysates using anti- CD44 antibody at 1/500 dilution. Positive control: Lane 1: HUVEC cell lysate Lane 2: HL-60 cell lysate Lane 3: Hela cell lysate Lane 4: SKOV-3 cell lysate
	Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. Counter stained with hematoxylin.
	Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD44 antibody. Counter stained with hematoxylin.
	Fig4: Flow cytometric analysis of Hela cells with CD44 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.