CD44 Mouse Monoclonal Antibody [A7-1]
cat.: EM010805
| Product Type: | Mouse monoclonal IgM, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A7-1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 81 kDa |
| Isotype: | IgM |
| Immunogen: | Synthetic peptide within human CD44 aa 100-200. |
| Positive control: | HUVEC cell lysate, HL-60 cell lysate, Hela cell lysate, SKOV-3 cell lysate, human tonsil tissue, human colon carcinoma tissue, Hela. |
| Subcellular location: | Cell membrane |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:200 1:100-1:200 |
| Uniprot #: | SwissProt: P16070 Human |
| Alternative names: | LHR BA-1 CD 44 CD44 CD44 antigen CD44 molecule (Indian blood group) CD44 molecule CD44_HUMAN CDw44 Cell surface glycoprotein CD44 chondroitin sulfate proteoglycan 8 CSPG8 ECMR-III Epican Extracellular matrix receptor III GP90 lymphocyte homing/adhesion receptor HCELL hematopoietic cell E- and L-selectin ligand Heparan sulfate proteoglycan Hermes antigen homing function and Indian blood group system HSA HUTCH-I HUTCH1 Hyaluronate receptor IN INLU-related p80 Glycoprotein MC56 MDU2 MDU3 MGC10468 MIC4 MUTCH1 PGP-1 PGP-I PGP1 Phagocytic glycoprotein 1 Phagocytic glycoprotein I Soluble CD44 |
Images
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Fig1:
Western blot analysis of CD44 on different lysates with Mouse anti-CD44 antibody (EM010805) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HUVEC cell lysate Lane 3: PANC-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 81 kDa Observed band size: 81 kDa Exposure time: 4 Seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM010805) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CD44 on different lysates using anti- CD44 antibody at 1/500 dilution. Positive control: Lane 1: HUVEC cell lysate Lane 2: HL-60 cell lysate Lane 3: Hela cell lysate Lane 4: SKOV-3 cell lysate |
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Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD44 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-CD44 antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of Hela cells with CD44 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.