Beta Catenin Mouse Monoclonal Antibody [A6-F8]
cat.: EM0306
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Monoclonal
Clone number: A6-F8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 85 kDa
Isotype: IgG1
Immunogen: Synthetic peptide (KLH-coupled) within human Beta-catenin aa 320-400.
Positive control: 293T cell lysate, A431 cell lysate, NCCIT cell lysate, SW480 cell lysate, HT-29 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, NCCIT, rat testis tissue, human liver tissue, human colon cancer tissue, MCF7.
Subcellular location: Cytoplasm, Nucleus
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:1,000
1:100-1:200
1:50-1:200
1:100
Uniprot #: SwissProt: P35222 Human | Q02248 Mouse | Q9WU82 Rat
Alternative names: β Catenin Beta catenin Beta-catenin Cadherin associated protein Catenin (cadherin associated protein), beta 1, 88kDa Catenin beta 1 Catenin beta-1 CATNB CHBCAT CTNB1_HUMAN CTNNB CTNNB1 DKFZp686D02253 FLJ25606 FLJ37923 OTTHUMP00000162082 OTTHUMP00000165222 OTTHUMP00000165223 OTTHUMP00000209288 OTTHUMP00000209289
Images
EM0306_1.jpg Fig1: Western blot analysis of Beta Catenin on different lysates with Mouse anti-Beta Catenin antibody (EM0306) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: A431 cell lysate
Lane 3: NCCIT cell lysate
Lane 4: SW480 cell lysate
Lane 5: HT-29 cell lysate
Lane 6: HCT 116 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: C6 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 85 kDa
Observed band size: 85 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
EM0306_2.jpg Fig2: ICC staining Beta-Catenin in NCCIT cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM0306_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin.
EM0306_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin.
EM0306_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin.
EM0306_6.jpg Fig6: Flow cytometric analysis of MCF7 cells labeling Beta Catenin.

Cells were fixed and permeabilized. Then stained with the primary antibody (EM0306, 1/100) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1126) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.