Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | A6-F8 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 85 kDa |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide (KLH-coupled) within human Beta-catenin aa 320-400. |
Positive control: | 293T cell lysate, A431 cell lysate, NCCIT cell lysate, SW480 cell lysate, HT-29 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, NCCIT, rat testis tissue, human liver tissue, human colon cancer tissue, MCF7. |
Subcellular location: | Cytoplasm, Nucleus |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:100-1:200 1:50-1:200 1:100 |
Uniprot #: | SwissProt: P35222 Human | Q02248 Mouse | Q9WU82 Rat |
Alternative names: | β Catenin Beta catenin Beta-catenin Cadherin associated protein Catenin (cadherin associated protein), beta 1, 88kDa Catenin beta 1 Catenin beta-1 CATNB CHBCAT CTNB1_HUMAN CTNNB CTNNB1 DKFZp686D02253 FLJ25606 FLJ37923 OTTHUMP00000162082 OTTHUMP00000165222 OTTHUMP00000165223 OTTHUMP00000209288 OTTHUMP00000209289 |
Fig1:
Western blot analysis of Beta Catenin on different lysates with Mouse anti-Beta Catenin antibody (EM0306) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining Beta-Catenin in NCCIT cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin. |
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin. | |
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin. | |
Fig6:
Flow cytometric analysis of MCF7 cells labeling Beta Catenin. Cells were fixed and permeabilized. Then stained with the primary antibody (EM0306, 1/100) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1126) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |