Chromogranin-A(C-term) Mouse Monoclonal Antibody [D11-2]
cat.: EM0407-24
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC
Clonality: Monoclonal
Clone number: D11-2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 86 kDa
Isotype: IgG2a
Immunogen: Synthetic peptide within human Chromogranin-A aa 415-457/457.
Positive control: PC-12, Hela, LOVO, PANC-1, rat small intestine tissue, rat pancreas tissue, mouse pancreas tissue.
Subcellular location: Secreted. Secretory vesicle lumen.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P10645 Human | P26339 Mouse | P10354 Rat
Alternative names: beta Granin betagranin (N-terminal fragment of chromogranin A) catestatin CgA CHG A Chga chromofungin Chromogranin A Chromogranin A parathyroid secretory protein 1 Chromogranin A precursor ChromograninA CMGA_HUMAN ER-37 Pancreastatin Parastatin Parathyroid secretory protein 1 Pituitary secretory protein I Secretory protein I SP I SP-I SP1 SPI Vasostatin Vasostatin I Vasostatin II
Images
EM0407-24_1.jpg Fig1: Western blot analysis of Chromogranin A on PC-12 using anti-Chromogranin A antibody at 1/500 dilution.
EM0407-24_2.jpg Fig2: ICC staining Chromogranin A(red) in Hela cells. The nuclear counter stain is DAPI(blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM0407-24_3.jpg Fig3: ICC staining Chromogranin A(red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM0407-24_4.jpg Fig4: ICC staining Chromogranin A(red) in PANC-1 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM0407-24_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
EM0407-24_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
EM0407-24_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.