Chromogranin-A(C-term) Mouse Monoclonal Antibody [D11-2]
cat.: EM0407-24
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | D11-2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG2a |
| Immunogen: | Synthetic peptide within human Chromogranin-A aa 415-457/457. |
| Positive control: | PC-12 cell lysates, PC-12, Hela, LOVO, PANC-1, mouse pancreas tissue, rat pancreas tissue, rat small intestine tissue. |
| Subcellular location: | Secreted. Secretory vesicle lumen. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P10645 Human | P26339 Mouse | P10354 Rat |
| Alternative names: | beta Granin betagranin (N-terminal fragment of chromogranin A) catestatin CgA CHG A Chga chromofungin Chromogranin A Chromogranin A parathyroid secretory protein 1 Chromogranin A precursor ChromograninA CMGA_HUMAN ER-37 Pancreastatin Parastatin Parathyroid secretory protein 1 Pituitary secretory protein I Secretory protein I SP I SP-I SP1 SPI Vasostatin Vasostatin I Vasostatin II |
Images
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Fig1:
Western blot analysis of Chromogranin A on PC-12 cell lysates using anti-Chromogranin A antibody at 1/500 dilution. Predicted band size: 51 kDa Observed band size: 86 kDa |
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Fig2:
Immunocytochemistry analysis of PC-12 cells labeling Chromogranin-A(C-term) with Mouse anti-Chromogranin-A(C-term) antibody (EM0407-24) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Chromogranin-A(C-term) antibody (EM0407-24) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3: ICC staining Chromogranin A(red) in Hela cells. The nuclear counter stain is DAPI(blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Chromogranin A(red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining Chromogranin A(red) in PANC-1 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Mouse anti-Chromogranin-A(C-term) antibody (EM0407-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0407-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Mouse anti-Chromogranin-A(C-term) antibody (EM0407-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0407-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat small intestine tissue with Mouse anti-Chromogranin-A(C-term) antibody (EM0407-24) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0407-24) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.