E-Cadherin Mouse Monoclonal Antibody [A0-G11-2]
cat.: EM0502
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | A0-G11-2 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 97 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within mouse E-Cadherin aa 350-550. |
| Positive control: | SW480 cell lysate, A431 cell lysate, human liver tissue, mouse liver tissue, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:500-1:1,000 1:500 |
| Uniprot #: | SwissProt: P12830 Human | P09803 Mouse | Q9R0T4 Rat |
| Alternative names: | Arc 1 CADH1_HUMAN Cadherin 1 cadherin 1 type 1 E-cadherin Cadherin1 CAM 120/80 CD 324 CD324 CD324 antigen cdh1 CDHE E-Cad/CTF3 E-cadherin ECAD Epithelial cadherin epithelial calcium dependant adhesion protein LCAM Liver cell adhesion molecule UVO Uvomorulin |
Images
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Fig1:
Western blot analysis of E-Cadherin with anti-E-Cadherin antibody [A0-G11-2] (EM0502) at 1:1,000 dilution. Lane 1/2: Wild-type A431 whole cell lysate (20 µg). Lane 3/4: E-Cadherin fragment 1 knockdown A431 whole cell lysate (20 µg). Lane 5/6: E-Cadherin fragment 2 knockdown A431 whole cell lysate (20 µg). Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0502, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1/20,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (EM0502) at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 98 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0502) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-E-Cadherin antibody (EM0502) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0502) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Application: IF-Tissue Species: Mouse Site: colon Sample: Paraffin-embedded section Antibody concentration: 1/500 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.