Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | IF-Cell, IHC-P, FC |
Clonality: | Monoclonal |
Clone number: | 10-E10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 62 kDa |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide within Human Sequestosome-1 aa 42-91 / 440. |
Positive control: | Hela cell lysate, HepG2 cell lysate, HepG2, mouse spleen tissue, mouse prostate tissue, mouse pancreas tissue, Hela. |
Subcellular location: | Cytoplasm, Nucleus |
Recommended Dilutions:
IF-Cell IHC-P FC |
1:200 1:200-1:1,000 1:50 |
Uniprot #: | SwissProt: Q13501 Human | Q64337 Mouse | O08623 Rat |
Alternative names: | A170 DMRV EBI 3 associated protein of 60 kDa EBI 3 associated protein p60 EBI3 associated protein of 60 kDa EBI3 associated protein p60 EBI3-associated protein of 60 kDa EBIAP FTDALS3 MGC127197 ORCA OSF-6 Osi OSIL Oxidative stress induced like p60 p62 p62B Paget disease of bone 3 PDB 3 PDB3 Phosphotyrosine independent ligand for the Lck SH2 domain of 62 kDa Phosphotyrosine independent ligand for the Lck SH2 domain p62 Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa PKC-zeta-interacting protein Protein kinase C-zeta-interacting protein Sequestosome 1 Sequestosome-1 SQSTM 1 SQSTM_HUMAN Sqstm1 STAP STONE14 Ubiquitin binding protein p62 Ubiquitin-binding protein p62 ZIP 3 ZIP ZIP3 |
Fig1: ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig2: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin. | |
Fig3: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin. |
Fig5: Flow cytometric analysis of Hela cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody. |