Anti-OCT-4 antibody [A3-C11]
cat.: EM100306
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC
Clonality: Monoclonal
Clone number: A3-C11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A purified.
Molecular weight: 39 kDa
Isotype: IgG1
Immunogen: peptide
Positive control: NCCIT, D3, F9
Subcellular location: Nucleus
Recommended Dilutions:
  WB
  ICC

1:1,000-1:5,000
1:200
Uniprot #: Q01860
Alternative names: Octamer binding transcription factor 4 antibody MGC22487 antibody Oct 3 antibody Oct 4 antibody Oct-3 antibody Oct-4 antibody OCT3 antibody Oct4 antibody Octamer binding protein 3 antibody Octamer binding protein 4 antibody Octamer binding transcription factor 3 antibody Octamer-binding protein 3 antibody Octamer-binding protein 4 antibody Octamer-binding transcription factor 3 antibody OTF 3 antibody OTF 4 antibody OTF-3 antibody OTF3 antibody OTF4 antibody PO5F1_HUMAN antibody POU class 5 homeobox 1 antibody POU domain class 5 transcription factor 1 antibody POU domain transcription factor OCT4 antibody POU domain, class 5, transcription factor 1 antibody POU-type homeodomain-containing DNA-binding protein antibody POU5F2 antibody
Images
EM100306_1.jpg Fig1: Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution.
Positive control: Line 1: NCCIT Line2 :D3
EM100306_2.jpg Fig2: ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM100306_3.jpg Fig3: ICC staining of OCT-4 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.