EM100306

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, ICC
Clonality:	Monoclonal
Clone number:	A3-C11

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2 mg/mL.

Purification:

Protein A purified.

Molecular weight:

39 kDa

Isotype:

IgG1

Immunogen:

peptide

Positive control:

NCCIT, D3, F9

Subcellular location:

Nucleus

Recommended Dilutions:
WB
ICC

1:1,000-1:5,000
1:200

Uniprot #:

Q01860

Alternative names:

Octamer binding transcription factor 4 antibody MGC22487 antibody Oct 3 antibody Oct 4 antibody Oct-3 antibody Oct-4 antibody OCT3 antibody Oct4 antibody Octamer binding protein 3 antibody Octamer binding protein 4 antibody Octamer binding transcription factor 3 antibody Octamer-binding protein 3 antibody Octamer-binding protein 4 antibody Octamer-binding transcription factor 3 antibody OTF 3 antibody OTF 4 antibody OTF-3 antibody OTF3 antibody OTF4 antibody PO5F1_HUMAN antibody POU class 5 homeobox 1 antibody POU domain class 5 transcription factor 1 antibody POU domain transcription factor OCT4 antibody POU domain, class 5, transcription factor 1 antibody POU-type homeodomain-containing DNA-binding protein antibody POU5F2 antibody

	Fig1: Western blot analysis of OCT-4 on different cell lysates using anti- OCT-4 antibody at 1/1000 dilution. Positive control: Line 1: NCCIT Line2 :D3
	Fig2: ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining of OCT-4 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.