Oct4 Mouse Monoclonal Antibody [A3-C11]
cat.: EM100306
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | A3-C11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 39 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within full length human Oct4 protein. |
| Positive control: | NCCIT cell lysate, D3 cell lysate, NCCIT. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000-1:5,000 1:200 1:1000 |
| Uniprot #: | SwissProt: Q01860 Human |
| Alternative names: | Octamer binding transcription factor 4 MGC22487 Oct 3 Oct 4 Oct-3 Oct-4 OCT3 Oct4 Octamer binding protein 3 Octamer binding protein 4 Octamer binding transcription factor 3 Octamer-binding protein 3 Octamer-binding protein 4 Octamer-binding transcription factor 3 OTF 3 OTF 4 OTF-3 OTF3 OTF4 PO5F1_HUMAN POU class 5 homeobox 1 POU domain class 5 transcription factor 1 POU domain transcription factor OCT4 POU domain, class 5, transcription factor 1 POU-type homeodomain-containing DNA-binding protein POU5F1 |
Images
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Fig1:
Western blot analysis of Oct4 on different lysates with Mouse anti-Oct4 antibody (EM100306) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Oct4 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 39 kDa Observed band size: 42 kDa Exposure time: 6 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM100306) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Oct4 on different lysates using anti-Oct4 antibody at 1/1,000 dilution. Positive control: Lane 1: NCCIT cell lysate Lane 2: D3 cell lysate |
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Fig3: ICC staining of OCT-4 in NCCIT cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Flow cytometric analysis of NCCIT cells labeling Oct4. Cells were fixed and permeabilized. Then stained with the primary antibody (EM100306, 1:1000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.