Product Type: | Mouse monoclonal IgM, primary antibodies |
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Species reactivity: | Human, Mouse, Rat, Zebrafish, Rabbit |
Applications: | WB, IF-Cell |
Clonality: | Monoclonal |
Clone number: | 5-E10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein L affinity purified. |
Molecular weight: | Predicted band size: 36 kDa |
Isotype: | IgM |
Immunogen: | Synthetic peptide within human GAPDH aa 10-50. |
Positive control: | MDA-MB-231 cell lysate, HeLa cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, JAR cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse kidney tissue lysate, rat liver tissue lysate, F9, Hela, HepG2. |
Subcellular location: | Cytoplasm |
Recommended Dilutions:
WB IF-Cell |
1:5,000-1:10,000 1:200-1:500 |
Uniprot #: | SwissProt: P04406 Human | P16858 Mouse | P04797 Rat |
Alternative names: | 38 kDa BFA-dependent ADP-ribosylation substrate aging associated gene 9 protein Aging-associated gene 9 protein BARS-38 cb609 EC 1.2.1.12 Epididymis secretory sperm binding protein Li 162eP G3P_HUMAN G3PD G3PDH GAPD GAPDH Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase HEL-S-162eP KNC-NDS6 MGC102544 MGC102546 MGC103190 MGC103191 MGC105239 MGC127711 MGC88685 OCAS, p38 component OCT1 coactivator in S phase, 38-KD component peptidyl cysteine S nitrosylase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH wu:fb33a10 |
Fig1:
Western blot analysis of GAPDH on different lysates with Mouse anti-GAPDH antibody (EM1101) at 1/5,000 dilution. Lane 1: MDA-MB-231 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: MCF7 cell lysate (20 µg/Lane) Lane 4: SK-Br-3 cell lysate (20 µg/Lane) Lane 5: JAR cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: C2C12 cell lysate (20 µg/Lane) Lane 8: PC-12 cell lysate (20 µg/Lane) Lane 9: Mouse kidney tissue lysate (40 µg/Lane) Lane 10: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1101) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig4: ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |