Product Type: | Mouse monoclonal IgM, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat, Zebrafish, Rabbit |
Applications: | WB, ICC |
Clonality: | Monoclonal |
Clone number: | 5-E10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2 mg/mL. |
Purification: | Protein L affinity purified. |
Molecular weight: | 36 kDa |
Isotype: | IgM |
Immunogen: | Synthetic peptide within human GAPDH aa 10-50. |
Positive control: | HepG2, Hela, PC12, NIH/3T3, MCF-7, Rabbit liver, zebrafish, F9 |
Subcellular location: | Cytoplasm |
Recommended Dilutions:
WB ICC |
1:5,000-1:10,000 1:200-1:500 |
Uniprot #: | SwissProt: P04406 Human |
Alternative names: | 38 kDa BFA-dependent ADP-ribosylation substrate aging associated gene 9 protein Aging-associated gene 9 protein BARS-38 cb609 EC 1.2.1.12 Epididymis secretory sperm binding protein Li 162eP G3P_HUMAN G3PD G3PDH GAPD GAPDH Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase HEL-S-162eP KNC-NDS6 MGC102544 MGC102546 MGC103190 MGC103191 MGC105239 MGC127711 MGC88685 OCAS, p38 component OCT1 coactivator in S phase, 38-KD component peptidyl cysteine S nitrosylase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH wu:fb33a10 |
![]() |
Fig1:
Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: PC12 Lane 4: NIH/3T3 Lane 5: MCF-7 Lane 6: Rabbit liver Lane 7: Zebrafish |
![]() |
Fig2: ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
![]() |
Fig3: ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
![]() |
Fig4: ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |