GAPDH Mouse Monoclonal Antibody [5-E10]
cat.: EM1101
Product Type: Mouse monoclonal IgM, primary antibodies
Species reactivity: Human, Mouse, Rat, Zebrafish, Rabbit
Applications: WB, ICC
Clonality: Monoclonal
Clone number: 5-E10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein L affinity purified.
Molecular weight: 36 kDa
Isotype: IgM
Immunogen: Synthetic peptide within human GAPDH aa 10-50.
Positive control: HepG2, Hela, PC12, NIH/3T3, MCF-7, Rabbit liver, zebrafish, F9
Subcellular location: Cytoplasm
Recommended Dilutions:
  WB
  ICC

1:5,000-1:10,000
1:200-1:500
Uniprot #: SwissProt: P04406 Human
Alternative names: 38 kDa BFA-dependent ADP-ribosylation substrate aging associated gene 9 protein Aging-associated gene 9 protein BARS-38 cb609 EC 1.2.1.12 Epididymis secretory sperm binding protein Li 162eP G3P_HUMAN G3PD G3PDH GAPD GAPDH Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase HEL-S-162eP KNC-NDS6 MGC102544 MGC102546 MGC103190 MGC103191 MGC105239 MGC127711 MGC88685 OCAS, p38 component OCT1 coactivator in S phase, 38-KD component peptidyl cysteine S nitrosylase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH wu:fb33a10
Images
EM1101_1.jpg Fig1: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela
Lane 3: PC12
Lane 4: NIH/3T3
Lane 5: MCF-7
Lane 6: Rabbit liver
Lane 7: Zebrafish
EM1101_2.jpg Fig2: ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1101_3.jpg Fig3: ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1101_4.jpg Fig4: ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.