EM1101

Product Type:	Mouse monoclonal IgM, primary antibodies
Species reactivity:	Human, Mouse, Rat, Zebrafish, Rabbit
Applications:	WB, ICC
Clonality:	Monoclonal
Clone number:	5-E10

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2 mg/mL.

Purification:

Protein L affinity purified.

Molecular weight:

36 kDa

Isotype:

IgM

Immunogen:

Synthetic peptide within human GAPDH aa 10-50.

Positive control:

HepG2, Hela, PC12, NIH/3T3, MCF-7, Rabbit liver, zebrafish, F9

Subcellular location:

Cytoplasm

Recommended Dilutions:
WB
ICC

1:5,000-1:10,000
1:200-1:500

Uniprot #:

SwissProt: P04406 Human

Alternative names:

38 kDa BFA-dependent ADP-ribosylation substrate aging associated gene 9 protein Aging-associated gene 9 protein BARS-38 cb609 EC 1.2.1.12 Epididymis secretory sperm binding protein Li 162eP G3P_HUMAN G3PD G3PDH GAPD GAPDH Glyceraldehyde 3 phosphate dehydrogenase Glyceraldehyde-3-phosphate dehydrogenase HEL-S-162eP KNC-NDS6 MGC102544 MGC102546 MGC103190 MGC103191 MGC105239 MGC127711 MGC88685 OCAS, p38 component OCT1 coactivator in S phase, 38-KD component peptidyl cysteine S nitrosylase GAPDH Peptidyl-cysteine S-nitrosylase GAPDH wu:fb33a10

	Fig1: Western blot analysis of GAPDH on different cell lysates using anti-GAPDH antibody at 1/5000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: PC12 Lane 4: NIH/3T3 Lane 5: MCF-7 Lane 6: Rabbit liver Lane 7: Zebrafish
	Fig2: ICC staining GAPDH in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining GAPDH in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: ICC staining GAPDH in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.