Anti-EP-CAM antibody [3-E1-E2]
cat.: EM1111
Product Type: Mouse monoclonal IgM, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: 3-E1-E2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein L affinity purified.
Molecular weight: 35 kDa
Isotype: IgM
Immunogen: recombinant protein
Positive control: Hela, NIH/3T3, MCF-7, A431, SW1990, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue
Subcellular location: Cell membrane
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000-1:2,000
1:100-1:200
1:200-1:500
1:100-1:200
Uniprot #: SwissProt: P16422 Human
Alternative names: 17 1A 323/A3 Adenocarcinoma associated antigen Adenocarcinoma-associated antigen Antigen identified by monoclonal AUA1 AUA1 CD326 CD326 antigen Cell surface glycoprotein Trop 1 Cell surface glycoprotein Trop 2 Cell surface glycoprotein Trop-1 CO 17A CO17 1A CO17A DIAR5 EGP 2 EGP EGP2 EGP314 EGP40 Ep CAM Ep-CAM EPCAM EPCAM_HUMAN EpCAM1 Epithelial cell adhesion molecule Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) Epithelial cell surface antigen Epithelial cellular adhesion molecule Epithelial glycoprotein 1 Epithelial glycoprotein 314 Epithelial glycoprotein ESA GA733 1 GA733 2 GA733-2 gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein gp4 hEGP 2 hEGP314 HNPCC8 Human epithelial glycoprotein 2 KS 1/4 antigen KS1/4 KSA Ly74 Lymphocyte antigen 74 M1S 1 M1S2 M4S1 Major gastrointestinal tumor associated protein GA733 2 Major gastrointestinal tumor-associated protein GA733-2 mEGP314 Membrane component chromosome 4 surface marker (35kD glycoprotein) Membrane component, chromosome 4, surface marker 1 Membrane component, chromosome 4, surface marker MIC18 MK 1 Protein 289A TACD1 TACSTD1 TROP1 Tumor associated calcium signal transducer 1 Tumor associated calcium signal transducer 2 precursor Tumor-associated calcium signal transducer 1
Images
EM1111_1.jpg Fig1: Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: MCF-7
EM1111_2.jpg Fig2: ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1111_3.jpg Fig3: ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1111_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
EM1111_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
EM1111_6.jpg Fig6: ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1111_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
EM1111_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.