EM1111

Product Type:	Mouse monoclonal IgM, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IF-Cell, IHC-P
Clonality:	Monoclonal
Clone number:	3-E1-E2

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2ug/ul

Purification:

Protein L affinity purified.

Molecular weight:

Predicted band size: 35 kDa

Isotype:

IgM

Immunogen:

Synthetic peptide within human EP-CAM aa 24-265 (Extracellular).

Positive control:

Hela cell lysate, NIH/3T3 cell lysate, MCF-7 cell lysate, D3, A431, human colon carcinoma tissue, human kidney tissue, human breast carcinoma tissue, mouse kidney tissue.

Subcellular location:

Cell membrane

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1,000-1:2,000
1:100-1:200
1:200-1:500

Uniprot #:

SwissProt: P16422 Human | Q99JW5 Moses

Alternative names:

17 1A 323/A3 Adenocarcinoma associated antigen Adenocarcinoma-associated antigen Antigen identified by monoclonal AUA1 AUA1 CD326 CD326 antigen Cell surface glycoprotein Trop 1 Cell surface glycoprotein Trop 2 Cell surface glycoprotein Trop-1 CO 17A CO17 1A CO17A DIAR5 EGP 2 EGP EGP2 EGP314 EGP40 Ep CAM Ep-CAM EPCAM EPCAM_HUMAN EpCAM1 Epithelial cell adhesion molecule Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) Epithelial cell surface antigen Epithelial cellular adhesion molecule Epithelial glycoprotein 1 Epithelial glycoprotein 314 Epithelial glycoprotein ESA GA733 1 GA733 2 GA733-2 gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein gp4 hEGP 2 hEGP314 HNPCC8 Human epithelial glycoprotein 2 KS 1/4 antigen KS1/4 KSA Ly74 Lymphocyte antigen 74 M1S 1 M1S2 M4S1 Major gastrointestinal tumor associated protein GA733 2 Major gastrointestinal tumor-associa......

	Fig1: Western blot analysis of EpCAM on different lysates using anti- EpCAM antibody at 1/1000 dilution. Positive control: Lane 1: Hela cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: MCF-7 cell lysate
	Fig2: ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using EpCAM antibody. Counter stained with hematoxylin.
	Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EpCAM antibody. Counter stained with hematoxylin.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using EpCAM antibody. Counter stained with hematoxylin.