PCNA Mouse Monoclonal Antibody [A6-G11]
cat.: EM111201
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: A6-G11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 29 kDa
Isotype: IgG1
Immunogen: Recombinant protein within human PCNA aa 1-261.
Positive control: L929, MCF-7, PC12, Raji, F9, A549, Hela ,human tonsil tissue, human gastric tumor tissue, mouse large intestine, mouse spleen tissue
Subcellular location: Nucleus
Recommended Dilutions:
  WB
  IHC-P
  FC

1:1,000-1:2,000
1:200
1:100
Uniprot #: SwissProt: P12004 Human | P17918 Mouse | P04961 Rat
Alternative names: ATLD2 cb16 Cyclin DNA polymerase delta auxiliary protein etID36690.10 fa28e03 fb36g03 HGCN8729 MGC8367 Mutagen-sensitive 209 protein OTTHUMP00000030189 OTTHUMP00000030190 PCNA Pcna/cyclin PCNA_HUMAN PCNAR Polymerase delta accessory protein Proliferating cell nuclear antigen wu:fa28e03 wu:fb36g05
Images
EM111201_1.jpg Fig1: Western blot analysis of PCNA on different cell lysates using anti-PCNA antibody at 1/1000 dilution.
Positive control:
Line 1: L929
Line 2 :MCF-7
Line 3:PC12
Line 4:Raji
Line 5:F9
Line 6:A549
EM111201_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PCNA antibody. Counter stained with hematoxylin.
EM111201_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PCNA antibody. Counter stained with hematoxylin.
EM111201_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti-PCNA antibody. Counter stained with hematoxylin.
EM111201_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-PCNA antibody. Counter stained with hematoxylin.
EM111201_6.jpg Fig6: Flow cytometric analysis of Hela cells with PCNA antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.