GFAP Mouse Monoclonal Antibody [1-D4]
cat.: EM140707
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, IF-Tissue, IHC-Fr |
| Clonality: | Monoclonal |
| Clone number: | 1-D4 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within C-terminal human GFAP. |
| Positive control: | Human brain tissue lysate, rat brain tissue lysate, human cerebellum tissue lysate, rat cerebellum tissue lysate, A172, N2A, rat brain tissue, rat cerebellum tissue, Hela, rat hippocampus tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Tissue IHC-P IHC-Fr |
1:5,000 1:500 1:500-1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P14136 Human | P03995 Mouse | P47819 Rat |
| Alternative names: | wu:fb34h11 ALXDRD cb345 etID36982.3 FLJ42474 FLJ45472 GFAP GFAP_HUMAN gfapl Glial fibrillary acidic protein Intermediate filament protein wu:fk42c12 xx:af506734 zgc:110485 |
Images
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Fig1:
Western blot analysis of GFAP on different lysates with Mouse anti-GFAP antibody (EM140707) at 1/5,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Human cerebellum tissue lysate Lane 4: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM140707) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: IHC-Fr Species: Rat Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-GFAP antibody (EM140707) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM140707) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue with Mouse anti-GFAP antibody (EM140707) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM140707) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GFAP antibody (EM140707) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM140707) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-GFAP antibody (EM140707) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM140707) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunofluorescence analysis of paraffin-embedded rat cerebellum tissue labeling GFAP (EM140707) and NeuN (ET1602-12). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies GFAP (EM140707, Red) at 1/500 dilution and NeuN (ET1602-12, Green) at 1/50 dilution overnight at 4 ℃, washed with PBS. iFluor™ 488 conjugate-Goat anti-Rabbit IgG (HA1121) and iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) were used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.