Alpha 1 Acid Glycoprotein Mouse Monoclonal Antibody [A4-G2-A3]
cat.: EM1510-42
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | A4-G2-A3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human ORM1 aa 1-201 / 201. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, HCT 116 cell lysate, 293T cell lysate, NIH:OVCAR-3 cell lysate, human liver tissue lysate, rat liver tissue, human lung cancer tissue, human esophagus tissue, HepG2. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P02763 Human | Q60590 Mouse | P02764 Rat |
| Alternative names: | A1AG1_HUMAN AGP 1 AGP A AGP AGP1 Alpha 1 acid glycoprotein Alpha-1-acid glycoprotein 1 alpha-1-AGP Epididymis secretory sperm binding protein Li 153w glycoprotein, alpha-1-acid, of serum HEL S 153w OMD 1 ORM ORM1 Orosomucoid 1 Orosomucoid-1 |
Images
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Fig1:
Western blot analysis of Alpha 1 Acid Glycoprotein on different lysates with Mouse anti-Alpha 1 Acid Glycoprotein antibody (EM1510-42) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: THP-1 cell lysate (20 µg/Lane) Lane 4: HCT 116 cell lysate (20 µg/Lane) Lane 5: 293T cell lysate (20 µg/Lane) Lane 6: NIH:OVCAR-3 cell lysate (20 µg/Lane) Lane 7: Human liver tissue lysate (40 µg/Lane) Predicted band size: 24 kDa Observed band size: 37/45 kDa Exposure time: 1 minute 59 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1510-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Alpha 1 Acid Glycoprotein antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of HepG2 cells with Alpha 1 Acid Glycoprotein antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-Mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.