PD1 Blocking Mouse Monoclonal Antibody [3A11]
cat.: EM1701-17
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | ELISA, FC |
| Clonality: | Monoclonal |
| Clone number: | 3A11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 32 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human PD1 aa 24 – 170 (Extracellular). |
| Positive control: | Jurkat. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
ELISA FC |
1:1,000-1:5,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q15116 Human |
| Alternative names: | CD279 CD279 antigen hPD 1 hPD l hPD-1 hSLE1 PD 1 PD-1 PD1 PDCD 1 PDCD1 PDCD1_HUMAN Programmed cell death 1 Programmed cell death 1 protein Programmed cell death protein 1 Protein PD 1 Protein PD-1 SLEB2 Systemic lupus erythematosus susceptibility 2 |
Images
|
Fig1: Flow cytometric analysis of Jurkat cells with PD1 blocking antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
|
Fig2: Blockade of Receptor-ligand Interaction at the cellular level using PD1 transfected CHO cells. In a functional ELISA, 50ng of PD1 antibody will block 52% of the binding of PD-L1-Fc to PD1 transfected CHO cells. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.