Anti-PD1 Blocking antibody [3A11]
cat.: EM1701-17
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: ELISA, FC
Clonality: Monoclonal
Clone number: 3A11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G purified.
Molecular weight: 32 kDa
Isotype: IgG1
Immunogen: Recombinant protein
Positive control: Jurkat.
Subcellular location: Membrane.
Recommended Dilutions:
  ELISA
  FC

1:1,000-1:5,000
1:50-1:100
Uniprot #: SwissProt: Q15116 Human
Images
EM1701-17_1.jpg Fig1: Flow cytometric analysis of Jurkat cells with PD1 blocking antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
EM1701-17_2.jpg Fig2: Blockade of Receptor-ligand Interaction at the cellular level using PD1 transfected CHO cells. In a functional ELISA, 50ng of PD1 antibody will block 52% of the binding of PD-L1-Fc to PD1 transfected CHO cells.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.