EM1701-21

Product Type:	Mouse monoclonal IgG2a, primary antibodies
Species reactivity:	Human, Mouse
Applications:	WB, IHC-P, ICC, FC
Clonality:	Monoclonal
Clone number:	5-F10

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

2 mg/mL.

Purification:

Protein A affinity purified.

Molecular weight:

37 kDa

Isotype:

IgG2a

Immunogen:

Recombinant protein within Human AKR1C1 aa 180-323 / 323.

Positive control:

HepG2, human liver tissue, MCF-7, human pancreas tissue, mouse kidney tissue.

Subcellular location:

Cytoplasm.

Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:2,000-1:10,000
1:50-1:200
1:50-1:200
1:50-1:100

Uniprot #:

SwissProt: Q04828 Human

Alternative names:

2-dihydrobenzene-1 2-diol dehydrogenase 20 alpha (3 alpha) hydroxysteroid dehydrogenase 20 ALPHA HSD 20 alpha hydroxysteroid dehydrogenase 20-alpha-HSD 20-alpha-hydroxysteroid dehydrogenase 20ALPHAHSD 2ALPHAHSD AK1C1 AK1C1_HUMAN AKR1C1 Aldo keto reductase family 1 member C1 aldo-keto reductase C Aldo-keto reductase family 1 member C1 Aldo-keto reductase family, 1 member 1 C9 Chlordecone reductase homolog Chlordecone reductase homolog HAKRC DD1 DD1/DD2 DDH DDH1 Dihydrodiol dehydrogenase 1 Dihydrodiol dehydrogenase 1/2 dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase dihydrodiol dehydrogenase isoform DD1 Dihydrodiol dehydrogenase, type 1 H37 HAKRC HBAB Hepatic dihydrodiol dehydrogenase High affinity hepatic bile acid-binding protein High-affinity hepatic bile acid-binding protein Indanol dehydrogenase MBAB MGC8954 Trans-1 Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase Type II 3 alph......

	Fig1: Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Human liver tissue
	Fig2: ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
	Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
	Fig6: Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.