Product Type: | Mouse monoclonal IgG2a, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IHC-P, ICC, FC |
Clonality: | Monoclonal |
Clone number: | 5-F10 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2 mg/mL. |
Purification: | Protein A purified. |
Molecular weight: | 37 kDa |
Isotype: | IgG2a |
Immunogen: | Recombinant protein |
Positive control: | HepG2, human liver tissue, MCF-7, human pancreas tissue, mouse kidney tissue. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB ICC IHC-P FC |
1:2,000-1:10,000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | Q04828(Human) |
Alternative names: | 2-dihydrobenzene-1 antibody 2-diol dehydrogenase antibody 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody 20 ALPHA HSD antibody 20 alpha hydroxysteroid dehydrogenase antibody 20-alpha-HSD antibody 20-alpha-hydroxysteroid dehydrogenase antibody 20ALPHAHSD antibody 2ALPHAHSD antibody AK1C1 antibody AK1C1_HUMAN antibody AKR1C1 antibody Aldo keto reductase family 1 member C1 antibody aldo-keto reductase C antibody Aldo-keto reductase family 1 member C1 antibody Aldo-keto reductase family, 1 member 1 antibody C9 antibody Chlordecone reductase homolog antibody Chlordecone reductase homolog HAKRC antibody DD1 antibody DD1/DD2 antibody DDH antibody DDH1 antibody Dihydrodiol dehydrogenase 1 antibody Dihydrodiol dehydrogenase 1/2 antibody dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase antibody dihydrodiol dehydrogenase isoform DD1 antibody Dihydrodiol dehydrogenase, type 1 antibody H37 antibody HAKRC antibody HBAB antibody Hepatic dihydrodiol dehydrogenase antibody High affinity hepatic bile acid-binding protein antibody High-affinity hepatic bile acid-binding protein antibody Indanol dehydrogenase antibody MBAB antibody MGC8954 antibody Trans-1 antibody Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase antibody Type II 3 alpha hydroxysteroid dehydrogenase antibody |
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Fig1:
Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution. Positive control: Lane 1: HepG2 Lane 2: Human liver tissue |
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Fig2: ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody. |