AKR1C1 Mouse Monoclonal Antibody [5-F10]
cat.: EM1701-21
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, ICC, FC
Clonality: Monoclonal
Clone number: 5-F10
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 37 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within Human AKR1C1 aa 180-323 / 323.
Positive control: HepG2, human liver tissue, MCF-7, human pancreas tissue, mouse kidney tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:2,000-1:10,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q04828 Human
Alternative names: 2-dihydrobenzene-1 2-diol dehydrogenase 20 alpha (3 alpha) hydroxysteroid dehydrogenase 20 ALPHA HSD 20 alpha hydroxysteroid dehydrogenase 20-alpha-HSD 20-alpha-hydroxysteroid dehydrogenase 20ALPHAHSD 2ALPHAHSD AK1C1 AK1C1_HUMAN AKR1C1 Aldo keto reductase family 1 member C1 aldo-keto reductase C Aldo-keto reductase family 1 member C1 Aldo-keto reductase family, 1 member 1 C9 Chlordecone reductase homolog Chlordecone reductase homolog HAKRC DD1 DD1/DD2 DDH DDH1 Dihydrodiol dehydrogenase 1 Dihydrodiol dehydrogenase 1/2 dihydrodiol dehydrogenase 1; 20-alpha (3-alpha)-hydroxysteroid dehydrogenase dihydrodiol dehydrogenase isoform DD1 Dihydrodiol dehydrogenase, type 1 H37 HAKRC HBAB Hepatic dihydrodiol dehydrogenase High affinity hepatic bile acid-binding protein High-affinity hepatic bile acid-binding protein Indanol dehydrogenase MBAB MGC8954 Trans-1 Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase Type II 3 alph......
Images
EM1701-21_1.jpg Fig1: Western blot analysis of AKR1C1 on different lysates using anti-AKR1C1 antibody at 1/2,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Human liver tissue
EM1701-21_2.jpg Fig2: ICC staining AKR1C1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-21_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
EM1701-21_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
EM1701-21_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKR1C1 antibody. Counter stained with hematoxylin.
EM1701-21_6.jpg Fig6: Flow cytometric analysis of HepG2 cells with AKR1C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.