EGFR Mouse Monoclonal Antibody [B6-E5-D9]
cat.: EM1701-22
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Green monkey |
| Applications: | WB, IHC-P, IF-Cell, FC(Intra) |
| Clonality: | Monoclonal |
| Clone number: | B6-E5-D9 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 134 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within human EGFR aa 900-1100. |
| Positive control: | A431 cell lysate, U-87 MG cell lysate, A549 cell lysate, LOVO, SW480, human tonsil tissue, human placenta tissue, mouse placenta tissue, human stomach cancer tissue, A431. |
| Subcellular location: | Cell membrane, Nucleus, cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC(Intra) |
1:5,000-1:10,000 1:500 1:50-1:200 1:5,000 |
| Uniprot #: | SwissProt: P00533 Human |
| Alternative names: | Avian erythroblastic leukemia viral (v erb b) oncogene homolog Cell growth inhibiting protein 40 Cell proliferation inducing protein 61 EGF R EGFR EGFR_HUMAN Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) Epidermal growth factor receptor erb-b2 receptor tyrosine kinase 1 ERBB ERBB1 Errp HER1 mENA NISBD2 Oncogen ERBB PIG61 Proto-oncogene c-ErbB-1 Receptor tyrosine protein kinase ErbB 1 Receptor tyrosine-protein kinase ErbB-1 SA7 Species antigen 7 Urogastrone v-erb-b Avian erythroblastic leukemia viral oncogen homolog wa2 Wa5 |
Images
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Fig1:
Western blot analysis of EGFR on different lysates with Mouse anti-EGFR antibody (EM1701-22) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: U-87 MG cell lysate Lane 3: A549 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 134 kDa Observed band size: 160 kDa Exposure time: Lane 1: 5 seconds; Lane 2-3: 43 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-22) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: A431 (Human cervix adenocarcinoma epithelial cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: EM1701-22, 1/500, overnight at 4℃. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature. Counterstain: Beta tubulin (ET1602-4, red), 1/100, overnight at 4℃. The Nuclear counterstain was DAPI (Blue). |
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Fig3:
Application: Flow Cytometry (Intra) Species: Human Sample: A431 (Human cervix adenocarcinoma epithelial cell) / Jurkat (Human T-lymphoblastic cells) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: EM1701-22(1/5,000, Red) compared with Mouse IgG1 Isotype Control (HA601336, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 15 minutes at room temperature. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EGFR antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-EGFR antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-EGFR antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.