Grp75 Mouse Monoclonal Antibody [6-A9]
cat.: EM1701-23
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | 6-A9 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within human Grp75 aa 101-150. |
| Positive control: | HepG2 cell lysate, L929 cell lysate, K562 cell lysate, NIH-3T3 cell lysate, A549, HepG2, SK-Br-3, rat epididymis tissue, mouse liver tissue, mouse oviduct tissue. |
| Subcellular location: | Mitochondrion. Nucleus. |
| Recommended Dilutions:
WB |
1:500-1:2,000 |
| Uniprot #: | SwissProt: P38646 Human | P38647 Mouse | P48721 Rat |
| Alternative names: | 75 kDa glucose regulated protein 75 kDa glucose-regulated protein CSA Glucose Regulated Protein Grp 75 GRP-75 GRP75 GRP75_HUMAN Heat shock 70 kDa protein 9 Heat shock 70kD protein 9 heat shock 70kDa protein 9 Heat shock 70kDa protein 9B Heat shock protein 74 kDa A Heat shock protein A Heat shock protein cognate 74 Hsc74 Hsp74 Hsp74a HSPA9 Hspa9a HSPA9B MGC4500 mitochondrial Mortalin 2 Mortalin Mortalin perinuclear Mortalin2 MOT 2 MOT MOT2 Mthsp70 p66 mortalin P66 MOT PBP74 Peptide binding protein 74 Peptide-binding protein 74 Stress 70 protein mitochondrial Stress 70 protein mitochondrial precursor Stress-70 protein |
Images
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Fig1:
Western blot analysis of Grp75 on different lysates with Mouse anti-Grp75 antibody (EM1701-23) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HepG2 cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: Mouse liver tissue lysate Lane 7: Rat liver tissue lysate Lane 8: Mouse kidney tissue lysate Lane 9: Rat kidney tissue lysate Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-23) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.