Anti-CD134/OX40 antibody [2H2]
cat.: EM1701-25
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC, FC
Clonality: Monoclonal
Clone number: 2H2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 55 kDa
Isotype: IgG1
Immunogen: Recombinant protein
Positive control: A549, Hela, HepG2, human tonsil tissue, human spleen tissue, mouse spleen tissue, HL-60.
Subcellular location: Membrane.
Recommended Dilutions:
  ICC
  IHC-P
  FC
  WB

1:50-1:200
1:50-1:200
1:50-1:100
1:500
Uniprot #: SwissProt: P43489 Human | P47741 Mouse | P15725 Rat
Alternative names: ACT 35 ACT35 ACT35 antigen ATC35 antigen CD 134 CD134 CD134 antigen IMD16 Lymphoid activation antigene ACT35 OX 40 OX40 OX40 antigen OX40 cell surface antigen OX40 homologue OX40L receptor TAX transcriptionally activated glycoprotein 1 receptor TAX transcriptionally-activated glycoprotein 1 receptor TNF receptor superfamily member 4 TNFRSF 4 TNFRSF4 TNR4_HUMAN Tumor necrosis factor receptor superfamily member 4 Txgp 1l Txgp1 Txgp1l
Images
EM1701-25_1.jpg Fig1: ICC staining CD134 (green) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-25_2.jpg Fig2: ICC staining CD134 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-25_3.jpg Fig3: ICC staining CD134 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-25_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD134 antibody. Counter stained with hematoxylin.
EM1701-25_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
EM1701-25_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD134 antibody. Counter stained with hematoxylin.
EM1701-25_7.jpg Fig7: Flow cytometric analysis of HL-60 cells with CD134 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.