PCSK9 Mouse Monoclonal Antibody [2F2]
cat.: EM1701-27
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse
Applications: IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: 2F2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein A affinity purified.
Molecular weight: 60 kDa
Isotype: IgG2b
Immunogen: Recombinant protein with Human PCSK9 1-201 / 692.
Positive control: PANC-1, LOVO, human liver tissue, human colon cancer tissue, human kidney tissue, Hela.
Subcellular location: Secreted.
Recommended Dilutions:
  IF-Cell
  IHC-P
  FC

1:50-1:100
1:50
1:50-1:100
Uniprot #: SwissProt: Q8NBP7 Human | Q80W65 Mouse
Alternative names: Convertase subtilisin/kexin type 9 preproprotein FH3 HCHOLA3 Hypercholesterolemia autosomal dominant 3 LDLCQ1 NARC 1 NARC-1 NARC1 Neural apoptosis regulated convertase 1 Neural apoptosis-regulated convertase 1 PC 9 PC9 PCSK 9 PCSK9 PCSK9_HUMAN Proprotein convertase 9 Proprotein convertase PC9 Proprotein convertase subtilisin/kexin type 9 PSEC0052 Subtilisin/kexin like protease PC9 Subtilisin/kexin-like protease PC9
Images
EM1701-27_1.jpg Fig1: ICC staining PCSK9 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-27_2.jpg Fig2: ICC staining PCSK9 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-27_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
EM1701-27_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon caner tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
EM1701-27_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCSK9 antibody. Counter stained with hematoxylin.
EM1701-27_6.jpg Fig6: Flow cytometric analysis of Hela cells with PCSK9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.