AFP Mouse Monoclonal Antibody [A5-G3]
cat.: EM1701-31
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, ICC
Clonality: Monoclonal
Clone number: A5-G3
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein A affinity purified.
Molecular weight: 68 kDa
Isotype: IgG1
Immunogen: Recombinant protein
Positive control: Human placenta tissue lysate, HepG2, Hela, MCF-7, human lung cancer tissue, human liver cancer tissue, human tonsil tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P02771 Human
Alternative names: Afp AFPD Alpha fetoglobulin Alpha fetoprotein Alpha fetoprotein precursor Alpha-1-fetoprotein Alpha-fetoglobulin Alpha-fetoprotein alpha-fetoprotein, Hereditary persistence of, included FETA FETA_HUMAN Hereditary persistence of alpha fetoprotein HPAFP
Images
EM1701-31_1.jpg Fig1: Western blot analysis of AFP on human placenta tissue lysate using anti-AFP antibody at 1/1,000 dilution.
EM1701-31_2.jpg Fig2: Western blot analysis of AFP on HepG2 cell lysate using anti-AFP antibody at 1/1,000 dilution.
EM1701-31_3.jpg Fig3: ICC staining AFP (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-31_4.jpg Fig4: ICC staining AFP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-31_5.jpg Fig5: ICC staining AFP (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-31_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
EM1701-31_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
EM1701-31_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-AFP antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.