B-Raf Mouse Monoclonal Antibody [F1-F4-E10]
cat.: EM1701-32
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | F1-F4-E10 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human B-Raf aa 49-239 / 766. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, HT-29 cell lysate, SK-MEL-28 cell lysate, HEK-293 cell lysate, SiHa cell lysate, COS-1 cell lysate, Vero cell lysate, PC-12 cell lysate, LOVO, SH-SY5Y, rat testis tissue, human testis tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue. |
| Subcellular location: | Nucleus, Cytoplasm, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:100-1:500 1:500-1:1,000 |
| Uniprot #: | SwissProt: P15056 Human | P28028 Mouse Entrez Gene: 114486 Rat |
| Alternative names: | FLJ95109 94 kDa B raf protein B raf 1 B Raf proto oncogene serine threonine protein kinase B Raf proto oncogene, serine/threonine kinase B RAF1 B-Raf proto-oncogene serine/threonine-protein kinase (p94) BRAF 1 BRAF BRAF_HUMAN BRAF1 cRmil MGC126806 MGC138284 Murine sarcoma viral (v-raf) oncogene homolog B1 Murine sarcoma viral v raf oncogene homolog B1 NS7 Oncogen BRAF oncogene BRAF1 p94 Proto-oncogene B-Raf Proto-oncogene c-Rmil RAFB 1 RAFB1 RMIL Serine/threonine-protein kinase B-raf v raf murine sarcoma viral oncogene homolog B v raf murine sarcoma viral oncogene homolog B1 v-Raf murine sarcoma viral oncogene homolog B1 |
Images
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Fig1:
Western blot analysis of B-Raf on different lysates with Mouse anti-B-Raf antibody (EM1701-32) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: K-562 cell lysate Lane 3: HT-29 cell lysate Lane 4: SK-MEL-28 cell lysate Lane 5: HEK-293 cell lysate Lane 6: SiHa cell lysate Lane 7: COS-1 cell lysate Lane 8: Vero cell lysate Lane 9: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-32) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining B Raf (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining B Raf (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-B-Raf antibody (EM1701-32) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-32) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-B-Raf antibody (EM1701-32) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-32) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-B-Raf antibody (EM1701-32) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-32) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-B Raf antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B Raf antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.