Insulin Mouse Monoclonal Antibody [5E1]
cat.: EM1701-33
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, ELISA |
| Clonality: | Monoclonal |
| Clone number: | 5E1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 12 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human Insulin aa 1-130. |
| Positive control: | Human pancreas tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
IHC-P ELISA |
1:2,000-1:10,000 1:5,000-1:20,000 |
| Uniprot #: | SwissProt: P01308 Human |
| Alternative names: | IDDM IDDM1 IDDM2 ILPR ins INS_HUMAN Insulin A chain Insulin B chain IRDN MODY10 Preproinsulin Proinsulin Proinsulin precursor |
Images
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Fig1:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-Insulin antibody (EM1701-33) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-33) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.