Product Type: | Mouse monoclonal IgM, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P, IF-Cell, FC |
Clonality: | Monoclonal |
Clone number: | 5F1 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein L affinity purified. |
Molecular weight: | Predicted band size: 21 kDa |
Isotype: | IgM |
Immunogen: | Recombinant full length human glucagon protein. |
Positive control: | LOVO, PANC-1, SH-SY5Y, mouse pancreas tissue, rat pancreas tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2000 1:50-1:200 1:50-1:200 1:50-1:100 |
Uniprot #: | SwissProt: P01275 Human | P55095 Mouse | P06883 Rat |
Alternative names: | GCG |
Fig1: ICC staining Glucagon (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig2: ICC staining Glucagon (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Glucagon (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin. |
Fig5: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin. | |
Fig6: Flow cytometric analysis of PANC-1 cells with Glucagon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |