Glucagon Mouse Monoclonal Antibody [5F1]
cat.: EM1701-34
Product Type: Mouse monoclonal IgM, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC, FC
Clonality: Monoclonal
Clone number: 5F1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein L affinity purified.
Molecular weight: 21 kDa, additional band 55kDa
Isotype: IgM
Immunogen: Recombinant protein
Positive control: Mouse kidney tissue lysate, LOVO, PANC-1, SH-SY5Y, mouse pancreas tissue, rat pancreas tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P01275 Human | P55095 Mouse | P06883 Rat
Alternative names: GCG
Images
EM1701-34_1.jpg Fig1: Western blot analysis of Glucagon on mouse kidney tissue lysate using anti-Glucagon antibody at 1/500 dilution.
EM1701-34_2.jpg Fig2: ICC staining Glucagon (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-34_3.jpg Fig3: ICC staining Glucagon (green) in SH-SY5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-34_4.jpg Fig4: ICC staining Glucagon (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-34_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.
EM1701-34_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Glucagon antibody. Counter stained with hematoxylin.
EM1701-34_7.jpg Fig7: Flow cytometric analysis of PANC-1 cells with Glucagon antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.