| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 5H1 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human CTHRC1 aa 1-243 / 243. |
| Positive control: | CTHRC1 recombinant protein, rat large intestine tissue, human kidney tissue, human placenta tissue. |
| Subcellular location: | Extracellular matrix. Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q96CG8 Human | Q9D1D6 Mouse | Q8CG08 Rat |
| Alternative names: | Collagen triple helix repeat containing 1 Collagen triple helix repeat containing protein 1 Collagen triple helix repeat-containing protein 1 CTHR1_HUMAN Cthrc1 NMTC1 protein Protein NMTC1 |
|
Fig1: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CTHRC1 antibody. Counter stained with hematoxylin. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CTHRC1 antibody. Counter stained with hematoxylin. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-CTHRC1 antibody. Counter stained with hematoxylin. |
|
Fig4:
Western blot analysis of CTHRC1 on different lysates with Rabbit anti-CTHRC1 antibody (EM1701-36) at 1/1,000 dilution. Lane 1: CTHRC1 recombinant protein lysate Lysates/proteins at 20 ng/Lane. Exposure time: 180s ; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-36, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 26.2 kDa Observed band size: 26 kDa |