CD47 Mouse Monoclonal Antibody [1A5]
cat.: EM1701-37
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 1A5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band 35 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein |
| Positive control: | NIH-3T3, SH-SY5Y, Mouse brain, Hela, K562, human pancreas tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q08722 Human | Q61735 Mouse |
| Alternative names: | Antigen identified by monoclonal 1D8 Antigenic surface determinant protein OA3 CD 47 Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) CD47 antigen CD47 glycoprotein CD47 molecule CD47_HUMAN IAP Integrin Associated Protein Integrin associated signal transducer Integrin-associated protein Leukocyte surface antigen CD47 MER 6 MER6 OA 3 OA3 OTTHUMP00000041152 OTTHUMP00000041153 Protein MER6 Rh related antigen Surface antigen identified by monoclonal 1D8 |
Images
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Fig1: Western blot analysis of CD47 on NIH-3T3 cell (1), Mouse brain (2), Hela cell (3) and K562 cell (4) lysates using anti-CD47 antibody at 1/500 dilution. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD47 antibody. Counter stained with hematoxylin. |
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Fig3: Flow cytometric analysis of SH-SY5Y cells with CD47 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.