CD47 Mouse Monoclonal Antibody [1A5]
cat.: EM1701-37
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 1A5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band 35 kDa
Isotype: IgG1
Immunogen: Recombinant protein
Positive control: NIH-3T3, SH-SY5Y, Mouse brain, Hela, K562, human pancreas tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q08722 Human | Q61735 Mouse
Alternative names: Antigen identified by monoclonal 1D8 Antigenic surface determinant protein OA3 CD 47 Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) CD47 antigen CD47 glycoprotein CD47 molecule CD47_HUMAN IAP Integrin Associated Protein Integrin associated signal transducer Integrin-associated protein Leukocyte surface antigen CD47 MER 6 MER6 OA 3 OA3 OTTHUMP00000041152 OTTHUMP00000041153 Protein MER6 Rh related antigen Surface antigen identified by monoclonal 1D8
Images
EM1701-37_1.jpg Fig1: Western blot analysis of CD47 on NIH-3T3 cell (1), Mouse brain (2), Hela cell (3) and K562 cell (4) lysates using anti-CD47 antibody at 1/500 dilution.
EM1701-37_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD47 antibody. Counter stained with hematoxylin.
EM1701-37_3.jpg Fig3: Flow cytometric analysis of SH-SY5Y cells with CD47 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.