Cardiac Troponin T Mouse Monoclonal Antibody [3-G6]
cat.: EM1701-39
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | 3-G6 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 36 kDa |
| Isotype: | IgG2b |
| Immunogen: | Synthetic peptide within huamn Cardiac Troponin T aa 45-89/298. |
| Positive control: | Human heart tissue lysates, mouse heart tissue, human heart tissue, mouse heart tissue. |
| Subcellular location: | Cytoskeleton, Cytosol. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:500 |
| Uniprot #: | SwissProt: P45379 Human | P50752 Mouse |
| Alternative names: | Cardiac muscle troponin T Cardiomyopathy dilated 1D (autosomal dominant) Cardiomyopathy hypertrophic 2 CMD1D CMH2 CMPD2 cTnT LVNC6 MGC3889 OTTHUMP00000033864 OTTHUMP00000033865 OTTHUMP00000033866 OTTHUMP00000033867 OTTHUMP00000033870 OTTHUMP00000218095 RCM3 TNNT 2 TNNT2 TNNT2_HUMAN TnTc Troponin T cardiac muscle Troponin T type 2 (cardiac) Troponin T type 2 cardiac Troponin T, cardiac muscle Troponin T2 cardiac |
Images
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Fig1: Western blot analysis of Cardiac Troponin T on human heart tissue lysate using anti- Cardiac Troponin T antibody at 1/1,000 dilution. |
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Fig2: ICC staining Cardiac Troponin T (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Cardiac Troponin T (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti- Cardiac Troponin T antibody. Counter stained with hematoxylin. |
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Fig5:
Immunofluorescence analysis of paraffin-embedded human heart tissue labeling Cardiac Troponin T with Mouse anti-Cardiac Troponin T antibody (EM1701-39) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (EM1701-39, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.