GRB2 Mouse Monoclonal Antibody [11-B11]
cat.: EM1701-41
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 11-B11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human GRB2 aa 21-217 / 217. |
| Positive control: | 293T cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue, rat testis tissue, rat brain tissue, MCF-7, HepG2. |
| Subcellular location: | Endosome, Nucleus, Golgi apparatus, Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P62993 Human | Q60631 Mouse | P62994 Rat |
| Alternative names: | Abundant SRC homology Adapter protein GRB2 ASH Ash protein EGFRBP GRB2 Epidermal growth factor receptor binding protein Epidermal growth factor receptor binding protein GRB2 GRB 2 GRB2 adapter protein Grb2 GRB2_HUMAN Grb3 3 Growth factor receptor bound protein 2 Growth factor receptor bound protein 3 Growth factor receptor-bound protein 2 HT027 MST084 MSTP084 NCKAP2 OTTHUMP00000166096 OTTHUMP00000166097 OTTHUMP00000166098 Protein ash SEM5 SH2/SH3 adapter GRB2 |
Images
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Fig1:
Western blot analysis of GRB2 on different lysates with Mouse anti-GRB2 antibody (EM1701-41) at 1/1,000 dilution. Lane 1: 293T cell lysate (30 µg/Lane) Lane 2: A431 cell lysate (30 µg/Lane) Lane 3: NIH/3T3 cell lysate (30 µg/Lane) Lane 4: PC-12 cell lysate (30 µg/Lane) Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-GRB2 antibody (EM1701-41) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-GRB2 antibody (EM1701-41) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-41) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRB2 antibody. Counter stained with hematoxylin. |
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Fig5: ICC staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: ICC staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.