Anti-GRB2 antibody [11-B11]
cat.: EM1701-41
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC
Clonality: Monoclonal
Clone number: 11-B11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 25 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human GRB2 aa 21-217 / 217.
Positive control: 293, MCF-7, HepG2, rat brain tissue, human colon cancer tissue,
Subcellular location: Endosome, Nucleus, Golgi apparatus.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:1,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P62993 Human | Q60631 Mouse | P62994 Rat
Alternative names: Abundant SRC homology Adapter protein GRB2 ASH Ash protein EGFRBP GRB2 Epidermal growth factor receptor binding protein Epidermal growth factor receptor binding protein GRB2 GRB 2 GRB2 adapter protein Grb2 GRB2_HUMAN Grb3 3 Growth factor receptor bound protein 2 Growth factor receptor bound protein 3 Growth factor receptor-bound protein 2 HT027 MST084 MSTP084 NCKAP2 OTTHUMP00000166096 OTTHUMP00000166097 OTTHUMP00000166098 Protein ash SEM5 SH2/SH3 adapter GRB2
Images
EM1701-41_1.jpg Fig1: Western blot analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.
EM1701-41_2.jpg Fig2: ICC staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-41_3.jpg Fig3: ICC staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-41_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
EM1701-41_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.