CD11b Mouse Monoclonal Antibody [4-6]
cat.: EM1701-42
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | IHC-P, IF-Cell, FC, WB |
| Clonality: | Monoclonal |
| Clone number: | 4-6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human CD11b aa 544-743 / 1,152. |
| Positive control: | TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, A431, LOVO, human tonsil tissue, human spleen tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC WB |
1:50-1:200 1:50-1:200 1:50-1:100 1:1,000 |
| Uniprot #: | SwissProt: P11215 Human |
| Alternative names: | antigen CD11b (p170) Antigen CD11b p170 CD11 antigen like family member B CD11 antigen-like family member B CD11b CD11b/CD18 CD49d Cell surface glycoprotein MAC-1 subunit alpha Complement component 3 receptor 3 subunit Complement Component Receptor 3 Alpha Complement receptor type 3, alpha subunit CR 3 alpha chain (CR3A) CR 3 alpha chain CR-3 alpha chain CR3 CR3A F730045J24Rik Integrin Alpha M Integrin alpha M chain Integrin alpha-M Integrin beta 2 alpha subunit Integrin subunit alpha M integrin, alpha M (complement component 3 receptor 3 subunit) ITAM_HUMAN ITGAM Leukocyte adhesion receptor MO1 Ly-40 MAC 1 Mac-1a MAC1 Mac1, alpha subunit MAC1A Macrophage antigen alpha polypeptide MGC117044 Mo1, alpha subunit MO1A Neutrophil adherence receptor alpha M subunit Neutrophil adherence receptor SLEB6 |
Images
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Fig1:
Western blot analysis of CD11b on different lysates with Mouse anti-CD11b antibody (EM1701-42) at 1/1,000 dilution. Lane 1: TF-1 cell lysate Lane 2: THP-1 cell lysate Lane 3: U-937 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 127 kDa Observed band size: 170 kDa Exposure time: 30 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining CD11b (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining CD11b (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD11b antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD11b antibody. Counter stained with hematoxylin. |
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Fig6: Flow cytometric analysis of THP-1 cells with CD11b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.