CD130 Mouse Monoclonal Antibody [B11-B9-A8]
cat.: EM1701-43
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, ICC
Clonality: Monoclonal
Clone number: B11-B9-A8
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 103 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human CD130 238-422 / 918.
Positive control: CD130 recombinant protein, Raji cell and mouse lung tissue lysates, HUVEC, LOVO, human prostate tissue, human liver tissue, human kidney tissue, human pancreas tissue.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P40189 Human
Alternative names: CD130 CD130 antigen CDw130 gp130 GP130 RAPS IL 6R beta IL-6 receptor subunit beta IL-6R subunit beta IL-6R-beta IL-6RB IL6 ST IL6RB_HUMAN IL6ST Interleukin 6 receptor subunit beta Interleukin receptor beta chain Interleukin-6 receptor subunit beta Interleukin-6 signal transducer Membrane glycoprotein 130 Membrane glycoprotein gp130 Oncostatin M receptor Oncostatin M receptor alpha subunit Oncostatin-M receptor subunit alpha
Images
EM1701-43_1.jpg Fig1: Western blot analysis of CD130 on CD130 recombinant protein using anti-CD130 antibody at 1/5,000 dilution.
EM1701-43_2.jpg Fig2: Western blot analysis of CD130 on Raji cell (1) and mouse lung tissue (2) lysates using anti-CD130 antibody at 1/500 dilution.
EM1701-43_3.jpg Fig3: ICC staining CD130 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-43_4.jpg Fig4: ICC staining CD130 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-43_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD130 antibody. Counter stained with hematoxylin.
EM1701-43_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD130 antibody. Counter stained with hematoxylin.
EM1701-43_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD130 antibody. Counter stained with hematoxylin.
EM1701-43_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD130 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.