Product Type: | Mouse monoclonal IgG2b, primary antibodies |
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Species reactivity: | Human, Mouse |
Applications: | WB, IHC-P, IF-Cell |
Clonality: | Monoclonal |
Clone number: | B11-B9-A8 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | Predicted band size: 104 kDa |
Isotype: | IgG2b |
Immunogen: | Recombinant protein within Human CD130 238-422 / 918. |
Positive control: | CD130 recombinant protein, Raji cell and mouse lung tissue lysates, HUVEC, LOVO, human prostate tissue, human liver tissue, human kidney tissue, human pancreas tissue. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P40189 Human | Q00560 Mouse |
Alternative names: | CD130 CD130 antigen CDw130 gp130 GP130 RAPS IL 6R beta IL-6 receptor subunit beta IL-6R subunit beta IL-6R-beta IL-6RB IL6 ST IL6RB_HUMAN IL6ST Interleukin 6 receptor subunit beta Interleukin receptor beta chain Interleukin-6 receptor subunit beta Interleukin-6 signal transducer Membrane glycoprotein 130 Membrane glycoprotein gp130 Oncostatin M receptor Oncostatin M receptor alpha subunit Oncostatin-M receptor subunit alpha |
Fig1: Western blot analysis of CD130 on CD130 recombinant protein using anti-CD130 antibody at 1/5,000 dilution. | |
Fig2: Western blot analysis of CD130 on Raji cell (1) and mouse lung tissue (2) lysates using anti-CD130 antibody at 1/500 dilution. | |
Fig3: ICC staining CD130 (green) in HUVEC cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining CD130 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CD130 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD130 antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD130 antibody. Counter stained with hematoxylin. | |
Fig8: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-CD130 antibody. Counter stained with hematoxylin. |