IL-6 Mouse Monoclonal Antibody [1-6]
cat.: EM1701-45
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC
Clonality: Monoclonal
Clone number: 1-6
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 24 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Mouse IL-6 aa 1-211 / 211.
Positive control: Recombinant protein lysates, PC-3M, Hela, NIH/3T3, mouse spleen tissue.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P05231 Human | P08505 Mouse | P20607 Rat
Alternative names: Interleukin BSF 2 B cell differentiation factor B cell stimulatory factor 2 B-cell stimulatory factor 2 BSF 2 BSF-2 BSF2 CDF CTL differentiation factor Hepatocyte stimulatory factor HGF HSF Hybridoma growth factor Hybridoma growth factor Interferon beta-2 IFN-beta-2 IFNB2 IL 6 IL-6 IL6 IL6_HUMAN Interferon beta 2 Interferon beta-2 Interleukin 6 Interleukin 6 (interferon beta 2) Interleukin BSF 2 Interleukin-6
Images
EM1701-45_1.jpg Fig1: Western blot analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution.
EM1701-45_2.jpg Fig2: ICC staining of IL-6 in PC-3M cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1701-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1701-45_3.jpg Fig3: ICC staining of IL-6 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1701-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1701-45_4.jpg Fig4: ICC staining of IL-6 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (EM1701-45, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
EM1701-45_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IL-6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-45, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.