IFNAR1 Mouse Monoclonal Antibody [A5-A3]
cat.: EM1701-46
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A5-A3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 64 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within human IFNAR1 aa 28-127 (Extracellular). |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, NCCIT, SHG-44, human lung tissue, human colon tissue, human skin tissue. |
| Subcellular location: | Endosome. Cell membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P17181 Human | P33896 Mouse |
| Alternative names: | Alpha type antiviral protein Antiviral protein, alpha-type Antiviral protein, beta-type AVP Beta type antiviral protein CRF2-1 Cytokine receptor class-II member 1 Cytokine receptor family 2 member 1 IFN alpha REC IFN alpha receptor IFN alpha/beta Receptor alpha IFN beta receptor IFN Interferon-beta receptor IFN-alpha/beta receptor 1 IFN-R-1 IFNAR Ifnar1 IFNBR IFRC INAR1_HUMAN Interferon (alpha beta and omega) receptor 1 interferon alpha and beta receptor subunit 1 Interferon alpha/beta receptor 1 Interferon alpha/beta receptor alpha chain Interferon beta receptor 1 interferon receptor 1 Interferon-alpha receptor Type I interferon receptor 1 |
Images
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Fig1:
Western blot analysis of IFNAR1 on different lysates with Mouse anti-IFNAR1 antibody (EM1701-46) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 64 kDa Observed band size: 64/90/130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-46) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining IFNAR1(red) in NCCIT cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining IFNAR1(green) in SHG-44 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti- IFNAR1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.