PLEKHH1 Mouse Monoclonal Antibody [3-E11]
cat.: EM1701-48
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 3-E11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 151 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human PLEKHH1 aa 1,059-1,364 / 1,364. |
| Positive control: | MCF-7, A431, SH-SY5Y, human kidney tissue, mouse kidney tissue. |
| Subcellular location: | Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:500 1:50-1:200 |
| Uniprot #: | SwissProt: Q9ULM0 Human | Q80TI1 Mouse |
| Alternative names: | KIAA1200 PH domain containing family H member 1 Pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 pleckstrin homology domain-containing family H member 1 |
Images
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Fig1:
Western blot analysis of PLEKHH1 on A431 (Human epidermoid carcinoma skin squamous cell) lysates with Mouse anti-PLEKHH1 antibody (EM1701-48) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-48, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 151.2 kDa Observed band size: 35 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.