Product Type: | Mouse monoclonal IgG1, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC-P |
Clonality: | Monoclonal |
Clone number: | 2D2 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | Predicted band size: 10 kDa |
Isotype: | IgG1 |
Immunogen: | Synthetic peptide within Human HNP-1 aa 1-50 / 94. |
Positive control: | Rat spleen tissue lysates, human bone marrow tissue, mouse bone marrow tissue, mouse spleen tissue, rat bone marrow tissue, rat spleen tissue. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:2,000-1:5,000 |
Uniprot #: | SwissProt: P59665 Human | P11477 Mouse |
Alternative names: | alpha 1 DEF1 DEF1_HUMAN DEFA1 DEFA1B DEFA2 Defensin 1 Defensin Defensin, alpha 1 Defensin, alpha 1, myeloid related sequence Defensin, alpha 2 HNP-1 HNP-2 HNP1 HP-1 HP-2 HP1 HP2 MRS Myeloid related sequence Neutrophil defensin 1 Neutrophil defensin 2 |
![]() |
Fig1:
Immunohistochemical analysis of paraffin-embedded human bone marrow tissue with Mouse anti-HNP-1 antibody (EM1701-52) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-52) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue with Mouse anti-HNP-1 antibody (EM1701-52) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-52) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-HNP-1 antibody (EM1701-52) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-52) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig4:
Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Mouse anti-HNP-1 antibody (EM1701-52) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-52) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig5:
Immunohistochemical analysis of paraffin-embedded rat bone marrow tissue with Mouse anti-HNP-1 antibody (EM1701-52) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-52) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig6:
Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Mouse anti-HNP-1 antibody (EM1701-52) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-52) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig7:
Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Mouse anti-HNP-1 antibody (EM1701-52) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-52) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
![]() |
Fig8: Western blot analysis of HNP-1 cell lysate on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution. |