Product Type: | Mouse monoclonal IgG1, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Monoclonal |
Clone number: | 4D1 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 2ug/ul |
Purification: | Protein G affinity purified. |
Molecular weight: | Predicted band size: 86 kDa |
Isotype: | IgG1 |
Immunogen: | Recombinant protein with Human ADAM17 aa 215-315 / 824. |
Positive control: | SH-SY-5Y, PC-3M, rat epididymis tissue. |
Subcellular location: | Membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: Q9H2U9 Human | O35227 Mouse | Q63180 Rat |
Fig1:
Western blot analysis of Gp83 on SH-SY-5Y cells lysates using anti-Gp83 antibody at 1/1,000 dilution. |
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Fig2: ICC staining Gp83 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Gp83 antibody. Counter stained with hematoxylin. |