MAO-A Mouse Monoclonal Antibody [5D1]
cat.: EM1701-54
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 5D1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 60 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human MAO-A aa 222-517 / 527. |
| Positive control: | SH-SY5Y cell lysate, SiHa cell lysate, rat brain tissue lysate, mouse brain tissue lysate, SH-SY-5Y. |
| Subcellular location: | Mitochondrion outer membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: P21397 Human | Q64133 Mouse | P21396 Rat |
| Alternative names: | Amine oxidase [flavin containing] A Amine oxidase [flavin-containing] A AOFA AOFA_HUMAN EC 1.4.3.4 MAO A MAO-A maoA Monoamine oxidase A Monoamine oxidase type A |
Images
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Fig1:
Western blot analysis of MAO-A on different lysates with Mouse anti-MAO-A antibody (EM1701-54) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (20 µg/Lane) Lane 2: SiHa cell lysate (20 µg/Lane) Lane 3: Rat brain tissue lysate (40 µg/Lane) Lane 4: Mouse brain tissue lysate (40 µg/Lane) Predicted band size: 60 kDa Observed band size: 70 kDa Exposure time: Lane 1-2: 46 seconds; Lane 3-4: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-54) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2: ICC staining Monoamine Oxidase A in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.