Bmi1 Mouse Monoclonal Antibody [A11-C11]
cat.: EM1701-55
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A11-C11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within mouse Bmi1 aa 1-324 / 324. |
| Positive control: | Hela, NIH-3T3, SH-SY-5Y, human colon cancer tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:200 1:200-1:400 |
| Uniprot #: | SwissProt: P35226 Human | P25916 Mouse |
| Alternative names: | B lymphoma Mo MLV insertion region (mouse) B lymphoma Mo MLV insertion region 1 homolog Bmi 1 BMI1 BMI1 polycomb ring finger oncogene BMI1_HUMAN Flvi 2/bmi 1 FLVI2/BMI1 MGC12685 Murine leukemia viral (bmi 1) oncogene homolog Oncogene BMI 1 PCGF 4 PCGF4 Polycomb complex protein BMI 1 Polycomb complex protein BMI-1 Polycomb group protein Bmi1 Polycomb group ring finger 4 Polycomb group RING finger protein 4 RING finger protein 51 RNF 51 RNF51 |
Images
|
Fig1: Western blot analysis of Bmi1 on Hela (1) and NIH-3T3 (2) using anti-Bmi1 antibody at 1/500 dilution. |
|
Fig2: ICC staining Bmi1 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.