Bmi1 Mouse Monoclonal Antibody [A11-C11]
cat.: EM1701-55
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IHC-P, ICC
Clonality: Monoclonal
Clone number: A11-C11
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein affinity purified.
Molecular weight: 42 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within mouse Bmi1 aa 1-324 / 324.
Positive control: Hela, NIH-3T3, SH-SY-5Y, human colon cancer tissue, mouse spleen tissue.
Subcellular location: Cytoplasm. Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P

1:500-1:1,000
1:200
1:200-1:400
Uniprot #: SwissProt: P35226 Human | P25916 Mouse
Alternative names: B lymphoma Mo MLV insertion region (mouse) B lymphoma Mo MLV insertion region 1 homolog Bmi 1 BMI1 BMI1 polycomb ring finger oncogene BMI1_HUMAN Flvi 2/bmi 1 FLVI2/BMI1 MGC12685 Murine leukemia viral (bmi 1) oncogene homolog Oncogene BMI 1 PCGF 4 PCGF4 Polycomb complex protein BMI 1 Polycomb complex protein BMI-1 Polycomb group protein Bmi1 Polycomb group ring finger 4 Polycomb group RING finger protein 4 RING finger protein 51 RNF 51 RNF51
Images
EM1701-55_1.jpg Fig1: Western blot analysis of Bmi1 on Hela (1) and NIH-3T3 (2) using anti-Bmi1 antibody at 1/500 dilution.
EM1701-55_2.jpg Fig2: ICC staining Bmi1 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-55_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
EM1701-55_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.