Bmi1 Mouse Monoclonal Antibody [A11-C11]
cat.: EM1701-55
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | A11-C11 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 42 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within mouse Bmi1 aa 1-324 / 324. |
| Positive control: | Hela, NIH-3T3, SH-SY-5Y, human colon cancer tissue, mouse spleen tissue. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1,000 1:200 1:200-1:400 |
| Uniprot #: | SwissProt: P35226 Human | P25916 Mouse |
| Alternative names: | B lymphoma Mo MLV insertion region (mouse) B lymphoma Mo MLV insertion region 1 homolog Bmi 1 BMI1 BMI1 polycomb ring finger oncogene BMI1_HUMAN Flvi 2/bmi 1 FLVI2/BMI1 MGC12685 Murine leukemia viral (bmi 1) oncogene homolog Oncogene BMI 1 PCGF 4 PCGF4 Polycomb complex protein BMI 1 Polycomb complex protein BMI-1 Polycomb group protein Bmi1 Polycomb group ring finger 4 Polycomb group RING finger protein 4 RING finger protein 51 RNF 51 RNF51 |
Images
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Fig1:
Western blot analysis of Bmi1 on different lysates with Mouse anti-Bmi1 antibody (EM1701-55) at 1/2,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: 293T (Human embryonic kidney cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-55, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 36.9 kDa Observed band size: 37-42kDa |
|
Fig2: ICC staining Bmi1 (green) in SH-SY-5Y cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.