HRP Conjugated beta Tubulin Mouse Monoclonal Antibody [1-B11]
cat.: EM1701-59
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Zebrafish |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 1-B11 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human Beta tubulin aa 46-95 / 444. |
| Positive control: | K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human colon cancer tissue, human kidney tissue. |
| Subcellular location: | Cytoplasm, Cytoskeleton. |
| Recommended Dilutions:
WB IHC-P |
1:2,000-1:5,000 1:500-1:2,000 |
| Uniprot #: | SwissProt: P07437 Human | P99024 Mouse | P69897 Rat |
| Alternative names: | Beta 4 tubulin Beta 5 tubulin beta Ib tubulin Beta1 tubulin Class I beta tubulin M40 MGC117247 MGC16435 OK/SW cl.56 OK/SWcl.56 TBB5_HUMAN TUBB 1 TUBB 2 TUBB 5 TUBB TUBB1 TUBB2 TUBB5 tubulin beta 1 chain Tubulin beta 2 chain tubulin beta 5 chain Tubulin beta chain Tubulin beta class I tubulin beta polypeptide Tubulin beta-5 chain |
Images
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Fig1:
Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (EM1701-59) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 20 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-59) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-beta Tubulin antibody (EM1701-59, HRP) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-59, HRP) at 1/2,000 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-beta Tubulin antibody (EM1701-59, HRP) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-59, HRP) at 1/500 dilution for 1 hour at room temperature. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.