Calreticulin Mouse Monoclonal Antibody [7B2]
cat.: EM1701-61
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 7B2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 48 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant full length protein corresponding to Human Calretinin aa 1 to the C-terminus. |
| Positive control: | HepG2 cell lysate, HeLa cell lysate, HL-60 cell lysate, HeLa , human thyroid gland tissue, human prostate cancer tissue, human placenta tissue, Jurkat. |
| Subcellular location: | Endoplasmic reticulum. Secreted. Cytosol. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:250 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P27797 Human |
| Alternative names: | Autoantigen RO CALR CALR protein CALR_HUMAN Calregulin Calreticulin cC1qR CRP55 CRT CRTC Endoplasmic reticulum resident protein 60 Epididymis secretory sperm binding protein Li 99n ERp60 FLJ26680 grp60 HACBP HEL S 99n RO Sicca syndrome antigen A (autoantigen Ro; calreticulin) Sicca syndrome antigen A SSA |
Images
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Fig1:
Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (EM1701-61) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: HeLa cell lysate (20 µg/Lane) Lane 3: HL-60 cell lysate (20 µg/Lane) Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-61) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Calreticulin on different lysates with Mouse anti-Calreticulin antibody (EM1701-61) at 1/50,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si Calreticulin cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 48 kDa Observed band size: 55 kDa Exposure time: 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-61) at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of HeLa cells labeling Calreticulin with Mouse anti-Calreticulin antibody (EM1701-61) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Calreticulin antibody (EM1701-61) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Calreticulin antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Calreticulin antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of Jurkat cells with Calreticulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.