Calreticulin Mouse Monoclonal Antibody [7B2]
cat.: EM1701-61
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, ICC, FC
Clonality: Monoclonal
Clone number: 7B2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 55 kDa
Isotype: IgG1
Immunogen: Recombinant protein.
Positive control: HL-60, A431, HepG2, human thyroid gland tissue, human prostate cancer tissue, human placenta tissue, Jurkat.
Subcellular location: Endoplasmic reticulum. Secreted. Cytosol.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:2,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P27797 Human
Alternative names: Autoantigen RO CALR CALR protein CALR_HUMAN Calregulin Calreticulin cC1qR CRP55 CRT CRTC Endoplasmic reticulum resident protein 60 Epididymis secretory sperm binding protein Li 99n ERp60 FLJ26680 grp60 HACBP HEL S 99n RO Sicca syndrome antigen A (autoantigen Ro; calreticulin) Sicca syndrome antigen A SSA
Images
EM1701-61_1.jpg Fig1: Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.
EM1701-61_2.jpg Fig2: ICC staining Calreticulin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-61_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
EM1701-61_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
EM1701-61_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
EM1701-61_6.jpg Fig6: Flow cytometric analysis of Jurkat cells with Calreticulin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.