PI 3 Kinase p85 alpha Mouse Monoclonal Antibody [L3-D6]
cat.: EM1701-62
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | L3-D6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 84 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein within Human PI3-kinase p85 subunit alpha aa 19-219 / 724. |
| Positive control: | Raji cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HepG2, LOVO, rat brain tissue, human tonsil tissue, human colon cancer tissue, human placenta tissue. |
| Subcellular location: | Cytosol. Endoplasmic reticulum. Golgi apparatus. Nucleus. Plasma Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P IF-Tissue |
1:500-1:2,000 1:50-1:200 1:50-1:1,000 1:200 |
| Uniprot #: | SwissProt: P27986 Human | P26450 Mouse | Q63787 Rat |
| Alternative names: | GRB1 p85 alpha p85 P85A_HUMAN Phosphatidylinositol 3 kinase associated p 85 alpha Phosphatidylinositol 3 kinase regulatory 1 Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha Phosphatidylinositol 3-kinase regulatory subunit alpha Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) PI3 kinase p85 subunit alpha PI3-kinase regulatory subunit alpha PI3-kinase subunit p85-alpha PI3K PI3K regulatory subunit alpha Pik3r1 PtdIns 3 kinase p85 alpha PtdIns-3-kinase regulatory subunit alpha PtdIns-3-kinase regulatory subunit p85-alpha |
Images
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Fig1:
Western blot analysis of PI 3 Kinase p85 alpha on different lysates with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84 kDa Observed band size: 84 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-62) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining PI 3 Kinase p85 alpha (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PI 3 Kinase p85 alpha (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PI 3 Kinase p85 alpha antibody. Counter stained with hematoxylin. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded human lung tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunohistochemical analysis of paraffin-embedded mouse lung tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig10:
Immunohistochemical analysis of paraffin-embedded rat lung tissue with Mouse anti-PI 3 Kinase p85 alpha antibody (EM1701-62) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-62) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.