PTBP1 Mouse Monoclonal Antibody [9A1]
cat.: EM1701-63
| Product Type: | Mouse monoclonal IgG2a, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 9A1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG2a |
| Immunogen: | Recombinant protein within human PTBP1 aa 320-550. |
| Positive control: | Jurkat cell lysate, A431 cell lysate, K562 cell lysate, A549, 293T, SW480, human kidney tissue, human tonsil tissue, rat testis tissue, human colon cancer tissue, K562. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:5,000-1:10,000 1:50-1:100 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: P26599 Human | Q00438 Rat |
| Alternative names: | 57 kDa RNA binding protein PPTB 1 57 kDa RNA-binding protein PPTB-1 Heterogeneous nuclear ribonucleoprotein I Heterogeneous Nuclear Ribonucleoprotein Polypeptide I hnRNP I HNRNP-I HNRNPI HNRPI MGC10830 MGC8461 Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) Polypyrimidine Tract Binding Protein 1 Polypyrimidine tract binding protein Polypyrimidine tract-binding protein 1 pPTB PTB 1 PTB 2 PTB 3 PTB 4 PTB PTB T PTB1 PTB2 PTB3 PTB4 PTBP 1 PTBP1 PTBP1_HUMAN PTBT RNA Binding Protein |
Images
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Fig1:
Western blot analysis of PTBP1 on different lysates with Mouse anti-PTBP1 antibody (EM1701-63) at 1/5,000 dilution. Lane 1: Jurkat cell lysate Lane 2: A431 cell lysate Lane 3: K562 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-63) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining PTBP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PTBP1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PTBP1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PTBP1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTBP1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PTBP1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTBP1 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of K562 cells with PTBP1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-Mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.