BHMT Mouse Monoclonal Antibody [9E1]
cat.: EM1701-64
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC, FC
Clonality: Monoclonal
Clone number: 9E1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 45 kDa
Isotype: IgG1
Immunogen: Recombinant protein with Human BHMT aa 250-406 / 406.
Positive control: Human kidney tissue lysate, human liver tissue, HepG2, SiHa, rat kidney tissue, mouse liver tissue.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000-1:5,000
1:50
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q93088 Human | 329582 Mouse | O09171 Rat
Alternative names: Betaine homocysteine methyltransferase Betaine homocysteine S methyltransferase 1 Betaine homocysteine S methyltransferase Betaine--homocysteine S-methyltransferase 1 BHMT BHMT1 BHMT1_HUMAN EC 2.1.1.5 Epididymis secretory sperm binding protein Li 61p HEL S 61p
Images
EM1701-64_1.jpg Fig1: Western blot analysis of BHMT on human liver tissue and human kidney tissue lysate using anti-BHMT antibody at 1/1,000 dilution.
EM1701-64_2.jpg Fig2: ICC staining BHMT (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-64_3.jpg Fig3: ICC staining BHMT (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-64_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.
EM1701-64_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-BHMT antibody. Counter stained with hematoxylin.
EM1701-64_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-BHMT antibody. Counter stained with hematoxylin.
EM1701-64_7.jpg Fig7: Flow cytometric analysis of HepG2 cells with BHMT antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG1 was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.