Anti-Glucocorticoid Receptor alpha antibody [3F1]
cat.: EM1701-65
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human
Applications: WB, ICC, IHC-P, FC
Clonality: Monoclonal
Clone number: 3F1
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: 86 kDa
Isotype: IgG2a
Immunogen: Recombinant full length protein corresponding to Human Glucocorticoid Receptor alpha.
Positive control: Siha, A549, human spleen tissue, human breast cancer tissue.
Subcellular location: Nucleus. Cytoskeleton. Mitochondrion.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:1,000-1:5,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P04150 Human
Alternative names: GCCR GCR Glucocorticoid receptor alpha isoform Glucocorticoid receptor GR GRL NR3C1 Nuclear receptor subfamily 3 group C member 1
Images
EM1701-65_1.jpg Fig1: Western blot analysis of Glucocorticoid Receptor alpha on SiHa (1) and A549 (2) cell lysates using anti-Glucocorticoid Receptor alpha antibody at 1/5,000 dilution.
EM1701-65_2.jpg Fig2: ICC staining Glucocorticoid Receptor alpha (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-65_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
EM1701-65_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.
EM1701-65_5.jpg Fig5: Flow cytometric analysis of A549 cells with Glucocorticoid Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.