NFIB NF1B2 Mouse Monoclonal Antibody [1H2]
cat.: EM1701-67
Product Type: Mouse monoclonal IgG2a, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC, FC
Clonality: Monoclonal
Clone number: 1H2
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2 mg/mL.
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG2a
Immunogen: Recombinant protein within Human NFIB / NF1B2 aa 1-420 / 420.
Positive control: SH-SY5Y cell lysates, LO-2, rat heart tissue, human colon carcinoma tissue, human prostate tissue, human pancreas tissue, mouse liver tissue, SH-SY-5Y, rat liver tissue, human breast tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  ICC
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:1,000
1:50-1:100
Uniprot #: SwissProt: O00712 Human | P97863 Mouse
Entrez Gene: 29227 Rat
Alternative names: CCAAT Box Binding Transcription Factor CCAAT-box-binding transcription factor CTF HMGIC/NFIB NF-I/B NF1-B NF1B NF1B2 NFI-B NFI-RED Nfib NFIB_HUMAN NFIB2 NFIB3 Nuclear factor 1 B-type Nuclear factor 1/B Nuclear Factor 1B Nuclear Factor I B Nuclear factor I/B TGGCA Binding Protein TGGCA-binding protein TRANSCRIPTION FACTOR NFIB
Images
EM1701-67_1.jpg Fig1: Western blot analysis of NFIB/NF1B2 on SH-SY5Y cell lysates using anti-NFIB/NF1B2 antibody at 1/1,000 dilution.
EM1701-67_2.jpg Fig2: ICC staining NFIB/NF1B2 (green) in LO-2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
EM1701-67_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
EM1701-67_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-NFIB NF1B2 antibody (EM1701-67) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-67) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1701-67_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
EM1701-67_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
EM1701-67_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
EM1701-67_8.jpg Fig8: Flow cytometric analysis of SH-SY-5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
EM1701-67_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-NFIB NF1B2 antibody (EM1701-67) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-67) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1701-67_10.jpg Fig10: Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-NFIB NF1B2 antibody (EM1701-67) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-67) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.