NFIB / NF1B2 Mouse Monoclonal Antibody [1H5]
cat.: EM1701-69
| Product Type: | Mouse monoclonal IgG2b, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 1H5 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 47 kDa |
| Isotype: | IgG2b |
| Immunogen: | Recombinant protein within Human NFIB / NF1B2 aa 1-420 / 420. |
| Positive control: | SH-SY5Y,NIH/3T3,C6 rat heart tissue, human pancreas tissue, mouse liver tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: O00712 Human | P97863 Mouse Entrez Gene: 29227 Rat |
| Alternative names: | CCAAT Box Binding Transcription Factor CCAAT-box-binding transcription factor CTF HMGIC/NFIB NF-I/B NF1-B NF1B NF1B2 NFI-B NFI-RED Nfib NFIB_HUMAN NFIB2 NFIB3 Nuclear factor 1 B-type Nuclear factor 1/B Nuclear Factor 1B Nuclear Factor I B Nuclear factor I/B TGGCA Binding Protein TGGCA-binding protein TRANSCRIPTION FACTOR NFIB |
Images
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Fig1:
Western blot analysis of NFIB / NF1B2 on different lysates with Mouse anti-NFIB / NF1B2 antibody (EM1701-69) at 1/2,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate Lane 3: C6 (Rat glioma cell) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: EM1701-69, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 47.4 kDa Observed band size: 45-65 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.