NFIB / NF1B2 Mouse Monoclonal Antibody [1H5]
cat.: EM1701-69
Product Type: Mouse monoclonal IgG2b, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Monoclonal
Clone number: 1H5
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 47 kDa
Isotype: IgG2b
Immunogen: Recombinant protein within Human NFIB / NF1B2 aa 1-420 / 420.
Positive control: SH-SY5Y, rat heart tissue, human pancreas tissue, mouse liver tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O00712 Human | P97863 Mouse
Entrez Gene: 29227 Rat
Alternative names: CCAAT Box Binding Transcription Factor CCAAT-box-binding transcription factor CTF HMGIC/NFIB NF-I/B NF1-B NF1B NF1B2 NFI-B NFI-RED Nfib NFIB_HUMAN NFIB2 NFIB3 Nuclear factor 1 B-type Nuclear factor 1/B Nuclear Factor 1B Nuclear Factor I B Nuclear factor I/B TGGCA Binding Protein TGGCA-binding protein TRANSCRIPTION FACTOR NFIB
Images
EM1701-69_1.jpg Fig1: Western blot analysis of NFIB/NF1B2 on SH-SY5Y using anti-NFIB/NF1B2 antibody at 1/500 dilution.
EM1701-69_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
EM1701-69_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
EM1701-69_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-NFIB/NF1B2 antibody. Counter stained with hematoxylin.
EM1701-69_5.jpg Fig5: Flow cytometric analysis of SH-SY5Y cells with NFIB/NF1B2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.