Peroxiredoxin 2 Mouse Monoclonal Antibody [7F2]
cat.: EM1701-70
Product Type: Mouse monoclonal IgG1, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC, IF-Cell
Clonality: Monoclonal
Clone number: 7F2
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 2ug/ul
Purification: Protein G affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG1
Immunogen: Recombinant full length protein of Human PRDX2.
Positive control: PC-3M (Human prostate cancer cell) cell lysate,HEK-293 (Human embryonic kidney cell) cell lysate, LNCaP (Human prostate cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, Hep G2 (Human hepatocellular carcinoma cell) cell lysate, human liver carcinoma tissue, human thyroid carcinoma tissue, human prostate cancer tissue, human kidney tissue, human placenta tissue, Jurkat, PC-3.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IHC-P
  FC
  IF-Cell
1:2000-1:5000
1:50-1:1,000
1:50-1:200
1:50
Uniprot #: SwissProt: P32119 Human
Alternative names: Epididymis secretory sperm binding protein Li 2a HEL S 2a MGC4104 Natural killer cell enhancing factor B Natural killer cell-enhancing factor B Natural Killer Enhancing Factor B NKEF B NKEF-B NKEFB Peroxiredoxin-2 PRDX 2 PRDX2 PRDX2_HUMAN PrP PRX2 PRXII PTX1 TDPX1 Thiol Specific Antioxidant 1 Thiol specific antioxidant protein Thiol-specific antioxidant protein Thioredoxin Dependent Peroxide Reductase 1 Thioredoxin peroxidase 1 Thioredoxin-dependent peroxide reductase 1 Torin TPX1 TSA
Images
EM1701-70_1.jpg Fig1: Western blot analysis of Peroxiredoxin 2 on different lysates with Rabbit anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/5,000 dilution.

Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 2: LNCaP (Human prostate cancer cell) cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 4: SH-SY5Y (Human neuroblastoma cell) cell lysate
Lane 5: MCF7 (Human breast cancer cell) cell lysate
Lane 6: Hep G2 (Human hepatocellular carcinoma cell) cell lysate

Lysates/proteins at 15 µg/Lane.
Exposure time: 2 seconds; ECL: K1801


Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1701-70, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 22 kDa
Observed band size: 22 kDa
EM1701-70_2.jpg Fig2: Western blot analysis of Peroxiredoxin 2 on PC-3M (Human prostate cancer cell) with Rabbit anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/5,000 dilution.

Lysates/proteins at 10 µg/Lane.
Exposure time: 30 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: EM1701-70, 1/5,000 in 5% NFDM/TBST, 2 hours at room temperature
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1006), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 22 kDa
Observed band size: 22 kDa
EM1701-70_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-70) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1701-70_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-70) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
EM1701-70_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti- Peroxiredoxin 2 antibody. Counter stained with hematoxylin.
EM1701-70_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 2 antibody. Counter stained with hematoxylin.
EM1701-70_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti- Peroxiredoxin 2 antibody. Counter stained with hematoxylin.
EM1701-70_8.jpg Fig8: Immunocytochemistry analysis of PC-3 cells labeling Peroxiredoxin 2 with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/50 dilution.

Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-Peroxiredoxin 2 antibody (EM1701-70) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.