Peroxiredoxin 6 Mouse Monoclonal Antibody [7G1]
cat.: EM1701-73
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | 7G1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 25 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant full length protein of Human PRDX6. |
| Positive control: | PC-3M, K562, A431, SiHa, human liver tissue, human colon cancer tissue, human kidney tissue, human placenta tissue. |
| Subcellular location: | Cytoplasm. Lysosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:100-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: P30041 Human |
| Alternative names: | 1 Cys 1 Cys peroxiredoxin 1 Cys PRX 1 cysPrx 1-Cys peroxiredoxin 1-Cys PRX 24 kDa protein 9430088D19Rik AA690119 Acidic calcium independent phospholipase A2 Acidic calcium-independent phospholipase A2 aiPLA2 Antioxidant protein 2 AOP2 Aop2 rs3 Brp 12 Ciliary body glutathione peroxidase CP 3 EC 1.11.1.15 EC 1.11.1.7 EC 3.1.1. Epididymis secretory sperm binding protein Li 128m GPx HEL S 128m KIAA0106 Liver 2D page spot 40 Ltw4 Lvtw 4 MGC46173 mKIAA0106 Non selenium glutathione peroxidase Non-selenium glutathione peroxidase NSGPx ORF06 OTTHUMP00000032693 p29 Peroxiredoxin-6 Peroxiredoxin6 PHGPx Phospholipase A2 lysosomal PLA2 PRDX 6 Prdx5 PRDX6 Prdx6 rs3 PRDX6_HUMAN PRX Red blood cells page spot 12 Thiol specific antioxidant protein |
Images
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Fig1: Western blot analysis of PRDX6 on PC-3M (1) and K562 (2) using anti-PRDX6 antibody at 1/500 dilution. |
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Fig2: ICC staining PRDX6 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining PRDX6 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining PRDX6 (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PRDX6 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PRDX6 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRDX6 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PRDX6 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of PC-3M cells with PRDX6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.