ALDH4A1 Mouse Monoclonal Antibody [7E3]
cat.: EM1701-75
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 7E3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 62 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human ALDH4A1 aa 514-563 / 563. |
| Positive control: | SK-Br-3 cell lysate, HepG2 cell lysate, rat liver tissue, human liver tissue, human colon cancer tissue, human kidney tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IHC-P |
1:1,000-1:2,000 1:50-1:400 |
| Uniprot #: | SwissProt: P30038 Human | P0C2X9 Rat |
| Alternative names: | AL4A1_HUMAN aldehyde dehydrogenase 4 aldehyde dehydrogenase 4 family, member A1 Aldehyde dehydrogenase family 4 member A1 Aldehyde dehydrogenase, family 4, subfamily A, member 1 ALDH4 aldh4a1 Delta 1 pyrroline 5 carboxylate dehydrogenase, mitochondrial Delta-1-pyrroline-5-carboxylate dehydrogenase L-glutamate gamma-semialdehyde dehydrogenase mitochondrial mitochondrial delta-1-pyrroline 5-carboxylate dehydrogenase P5C dehydrogenase P5CD P5CDh P5CDhL P5CDhS Pyrroline-5-carboxylate dehydrogenase RP11 128M10.1 |
Images
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Fig1:
Western blot analysis of ALDH4A1 on different lysates with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: HepG2 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-75) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ALDH4A1 antibody. Counter stained with hematoxylin. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ALDH4A1 antibody (EM1701-75) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-75) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.