UGP2 Mouse Monoclonal Antibody [7H1]
cat.: EM1701-76
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | 7H1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human UGP2 aa 61-110 / 508. |
| Positive control: | Human liver tissue lysates, HepG2, NIH/3T3, rat liver tissue, human liver tissue, mouse heart tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:50-1:400 1:100 |
| Uniprot #: | SwissProt: Q16851 Human | Q91ZJ5 Mouse Entrez Gene: 289827 Rat |
| Alternative names: | pHC379 UDP glucose diphosphorylase UDP glucose pyrophosphorylase 1 UDP glucose pyrophosphorylase 2 UDP glucose pyrophosphorylase UDP-glucose pyrophosphorylase UDPG UDPGP 2 UDPGP UDPGP2 UGP 1 UGP 2 UGP1 Ugp2 UGPA_HUMAN UGPase 2 UGPase UGPP 1 UGPP 2 UGPP1 UGPP2 Uridyl diphosphate glucose pyrophosphorylase 1 Uridyl diphosphate glucose pyrophosphorylase 2 UTP glucose 1 phosphate uridyltransferase UTP glucose 1 phosphate uridylyltransferase 2 UTP glucose 1 phosphate uridylyltransferase UTP--glucose-1-phosphate uridylyltransferase |
Images
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Fig1:
Western blot analysis of UGP2 on human liver tissue lysates with Mouse anti-UGP2 antibody (EM1701-76) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 57 kDa Observed band size: 50 kDa Exposure time: 46 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-76) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HepG2 cells labeling UGP2 with Mouse anti-UGP2 antibody (EM1701-76) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-UGP2 antibody (EM1701-76) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Immunocytochemistry analysis of NIH/3T3 cells labeling UGP2 with Mouse anti-UGP2 antibody (EM1701-76) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-UGP2 antibody (EM1701-76) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-UGP2 antibody (EM1701-76) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-76) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-UGP2 antibody (EM1701-76) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-76) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-UGP2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.