UGP2 Mouse Monoclonal Antibody [7H1]
cat.: EM1701-76
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Monoclonal |
| Clone number: | 7H1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Protein G affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide within Human UGP2 aa 61-110 / 508. |
| Positive control: | Human liver tissue lysates, rat skeletal muscle tissue, human liver tissue, mouse heart tissue, LOVO. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:400 1:50-1:100 |
| Uniprot #: | SwissProt: Q16851 Human | Q91ZJ5 Mouse Entrez Gene: 289827 Rat |
| Alternative names: | pHC379 UDP glucose diphosphorylase UDP glucose pyrophosphorylase 1 UDP glucose pyrophosphorylase 2 UDP glucose pyrophosphorylase UDP-glucose pyrophosphorylase UDPG UDPGP 2 UDPGP UDPGP2 UGP 1 UGP 2 UGP1 Ugp2 UGPA_HUMAN UGPase 2 UGPase UGPP 1 UGPP 2 UGPP1 UGPP2 Uridyl diphosphate glucose pyrophosphorylase 1 Uridyl diphosphate glucose pyrophosphorylase 2 UTP glucose 1 phosphate uridyltransferase UTP glucose 1 phosphate uridylyltransferase 2 UTP glucose 1 phosphate uridylyltransferase UTP--glucose-1-phosphate uridylyltransferase |
Images
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Fig1: Western blot analysis of UGP2 on human liver tissue lysate using anti-UGP2 antibody at 1/200 dilution. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-UGP2 antibody. Counter stained with hematoxylin. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-UGP2 antibody (EM1701-76) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM1701-76) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-UGP2 antibody. Counter stained with hematoxylin. |
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Fig5: Flow cytometric analysis of LOVO cells with UGP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.