HSPA1L Mouse Monoclonal Antibody [7D1]
cat.: EM1701-78
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 7D1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 2ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 70 kDa |
| Isotype: | IgG1 |
| Immunogen: | Recombinant protein with Human HSPA1L aa 335-639 / 641. |
| Positive control: | 293 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, rat testis tissue, mouse testis tissue. |
| Subcellular location: | Cytosol. Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:10,000-1:50,000 1:50-1:100 |
| Uniprot #: | SwissProt: P34931 Human | P16627 Mouse | P55063 Rat |
| Alternative names: | Heat shock 70 kDa protein 1 Hom Heat shock 70 kDa protein 1 like Heat shock 70 kDa protein 1-Hom Heat shock 70 kDa protein 1-like Heat shock 70 kDa protein 1L Heat shock 70kD protein like 1 HS71L_HUMAN HSP70 1L HSP70 HOM HSP70-Hom HSPA1L hum70t Spermatid specific heat shock protein 70 |
Images
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Fig1:
Western blot analysis of HSPA1L on different lysates with Mouse anti-HSPA1L antibody (EM1701-78) at 1/5,000 dilution. Lane 1: 293 cell lysate (10 µg/Lane) Lane 2: Mouse testis tissue lysate (20 µg/Lane) Lane 3: Rat testis tissue lysate (20 µg/Lane) Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-78) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HSPA1L antibody. Counter stained with hematoxylin. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HSPA1L antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.